STABLE ISOTOPE LABELING TANDEM MASS SPETROMETRY (SILT) TO QUANTIFY PROTEIN

稳定同位素标记串联质谱法 (SILT) 定量蛋白质

• 批准号: 7953978
• 负责人: RANDALL J BATEMAN
• 金额: $ 0.93万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-02-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7953978
• 关键词: Aging; Alzheimer' s Disease; Amyloid beta-Protein; Animals; Computer Retrieval of Information on Scientific Projects Database; Disease; Funding; Grant; Homeostasis; Human; In Vitro; Inflammation; Institution; Mass Spectrum Analysis; Metabolic Clearance Rate; Methods; Pathogenesis; Production; Protein Biosynthesis; Proteins; Research; Research Personnel; Resources; Source; Speed; Stable Isotope Labeling; TANDEM; United States National Institutes of Health; base; biological systems; biomedical resource; in vivo; novel; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In all biological systems, protein amount is a function of the rate of production and clearance. The speed of a response to a disturbance in protein homeostasis is determined by turnover rate. Quantifying alterations in protein synthesis and clearance rates is vital to understanding disease pathogenesis (e.g., aging, inflammation). No methods currently exist for quantifying production and clearance rates of low-abundance (femtomole) proteins in vivo. We describe a novel, mass spectrometry-based method for quantitating low-abundance protein synthesis and clearance rates in vitro and in vivo in animals and humans. The utility of this method is demonstrated with amyloid-beta (Abeta), an important low-abundance protein involved in Alzheimer's disease pathogenesis.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在所有生物系统中，蛋白质的量是生产和清除速率的函数。对蛋白质稳态紊乱的反应速度由周转率决定。定量蛋白质合成和清除率的变化对于理解疾病发病机制至关重要（例如，老化、炎症）。目前还没有方法可以量化体内低丰度（毫微微摩尔）蛋白质的产生和清除率。我们描述了一种新的，质谱为基础的方法定量低丰度蛋白质的合成和清除率在体外和体内的动物和人类。这种方法的实用性证明与淀粉样蛋白β（Abeta），一个重要的低丰度蛋白参与阿尔茨海默氏病的发病机制。