Characterisation of herpes simplex virus morphogenesis by tracking the virus tegument through the secretory pathway

通过分泌途径追踪病毒外皮来表征单纯疱疹病毒形态发生

• 批准号: G0601605/1
• 负责人: Gill Elliott
• 金额: $ 207.17万
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Fellowship
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=G0601605%2F1
• 关键词: Characterisation; herpes; simplex; virus; morphogenesis

Herpes simplex virus (HSV) infects humans causing the sporadic appearance of cold sores or genital herpes. Serious complications of HSV infection, including encephalitis and meningitis, are potentially fatal in transplant patients, individuals undergoing chemotherapy, and newborn babies. To understand how HSV infection causes these diseases it is essential to find out how the virus grows in individual cells in the body. This proposal aims to study how single virus particles of HSV, that are less than one thousandth of a millimetre in diameter, assemble themselves inside the cell. The research will be carried out using viruses that have been modified to contain coloured markers that light up under a microscope. This allows individual viruses to be observed and followed in living cells. In this way, the journey that viruses make through the inside of a cell grown in culture in the laboratory can be traced using time-lapse microscopy. This approach will allow us to address the fundamental questions of how and where in a single cell the virus structure comes together. The resulting answers will then be applied to understanding virus infection in the human host.

单纯疱疹病毒（HSV）感染人类，引起散发的唇疱疹或生殖器疱疹。HSV感染的严重并发症，包括脑炎和脑膜炎，在移植患者、接受化疗的个体和新生儿中可能是致命的。要了解HSV感染是如何引起这些疾病的，就必须弄清楚病毒是如何在人体内的单个细胞中生长的。这项提议旨在研究HSV的单个病毒颗粒（直径小于千分之一毫米）是如何在细胞内组装的。这项研究将使用经过修饰的病毒来进行，这些病毒含有在显微镜下会发光的彩色标记。这样就可以在活细胞中观察和跟踪单个病毒。通过这种方式，病毒在实验室培养的细胞内部的旅程可以使用延时显微镜进行追踪。这种方法将使我们能够解决单个细胞中病毒结构如何以及在何处聚集在一起的基本问题。得到的答案将用于了解病毒在人类宿主中的感染。