Animal Hypoxia Core

动物缺氧核心

• 批准号: 8001453
• 负责人: Frank L. Powell
• 金额: $ 29.64万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8001453
• 关键词: Adult; Animals; Chronic; Core Facility; Data; Disease; Genotype; Health; Housing; Hypercapnia; Hypoxia; Individual; Laboratories; Lung diseases; Molecular; Physiological; Predisposition; Systems Biology; Training; animal resource; base; fly; improved

The primary objective of the Animal Hypoxia (Core C) is to provide standardized chronic exposures of sustained and intermittent hypoxia and/or hypercapnia to flies and mice used by all of the Projects, and to characterize the essential respiratory and cardiovascular phenotypes in the different species, genotypes, and exposures studied by the individual Projects. Standardizing exposure is vital for integrating the findings between the different Projects and optimizing data sharing. Centralizing this also insures quality control and dissemination of any results from individual Projects suggesting that the exposure protocols should be adjusted. Importantly, this centralization also conserves animals and resources to maximize the budget and animal sacrifice. Behavioral measurements are not included in this revised proposal but the larger Biospherix chambers, which house multiple animals and cages will be used for all chronic exposures to insure identical social interactions and conditioning. Phenotyping mice in the core also minimizes the cost and maximizes the information individual animal models in terms of the basic metabolic, respiratory and cardiovascular physiology that is necessary for all of the individual Projects. Given the challenges of maintaining disease free-breeding colonies for multiple lines of transgenic mice, it is most efficient and cost effective to make non-invasive and non-terminal descriptive measurements within a physiology core facility before distributing chronically exposed animals to individual laboratories for the actual experiments. Physiological data will be uploaded to a database developed with Systems Biology Core B for all the investigators to use so the projects can focus on the biology. Training in the physiological techniques used to phenotype the animals will also be provided by Core C to predoctoral students and postdoctoral fellows working in the individual Projects.

动物缺氧（核心 C）的主要目标是提供标准化的慢性暴露 对所有项目使用的果蝇和小鼠进行持续和间歇性缺氧和/或高碳酸血症，并 描述不同物种、基因型和不同物种的基本呼吸和心血管表型的特征 各个项目研究的风险。标准化暴露对于整合研究结果至关重要 不同项目之间的数据共享并优化。集中化还可以确保质量控制和 传播各个项目的任何结果，表明暴露协议应 调整。重要的是，这种集中化还可以保护动物和资源，从而最大限度地提高预算和 动物祭祀。行为测量不包括在这个修订后的提案中，但更大的 Biospherix 容纳多个动物和笼子的房间将用于所有慢性暴露，以确保相同的 社会互动和调节。在核心中对小鼠进行表型分析还可以最大限度地降低成本并最大化 个体动物模型在基本代谢、呼吸和心血管方面的信息 所有单个项目所必需的生理学。鉴于维持疾病的挑战 多系转基因小鼠的自由繁殖群体，是最有效且最具成本效益的 在分发之前在生理学核心设施内进行非侵入性和非终端描述性测量 长期将动物暴露在各个实验室进行实际实验。生理数据将 上传到使用 Systems Biology Core B 开发的数据库供所有研究人员使用，以便 项目可以专注于生物学。用于动物表型分析的生理技术培训 核心 C 也将提供给在个人工作的博士前学生和博士后研究员 项目。