Brain Mechanisms of Reproductive Behavior

生殖行为的大脑机制

• 批准号: 7931037
• 负责人: Donald W. Pfaff
• 金额: $ 11.13万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2011-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7931037
• 关键词: Address; Antisense DNA; Antisense Technology; Arts; Attention; Back; Behavior; Behavioral; Biodegradable microsphere; Biological Assay; Brain; Brain region; DNA Sequence; Data; Development; Environment; Equilibrium; Estrogens; Female; Gene Expression; Genes; Genetic; Genetic Techniques; Genomics; Grant; Head; Hormonal; Hormones; Humulus; Hypothalamic structure; Implant; Individual; Knock-out; Knockout Mice; Literature; Locomotion; Longevity; Lordosis; Messenger RNA; Methods; Microinjections; Microspheres; Mus; Neurosecretory Systems; Nuclear; One-Step dentin bonding system; Partner in relationship; Pattern; Performance; Pregnancy; Preoptic Areas; Progestins; Protein Isoforms; Proteins; Radial; Reagent; Relative (related person); Reproductive Behavior; Role; Running; Schedule; Sex Behavior; Site; Social Behavior; System; Techniques; Testing; Time; Upper arm; Viral; Viral Vector; Women' s Health; Work; approach behavior; base; knock-down; locked nucleic acid; male; novel; novel strategies; relating to nervous system; research study; response; tool

Great progress has been made in the analysis of hormonal, neural and genetic mechanisms underlying a simple sex behavior, lordosis. Now, it would be exciting to understand the flow of behaviors leading up to mating. Also, to explore the behavioral dispositions underlying these proceptive behaviors by the female. We have discovered active approach and pacing behaviors, previously thought to be absent, in female mice. New experiments with mice will allow for 'state of the art' genetic elucidation. (Aim I.): To finish carefully characterizing these locomotor, approach, proceptive and pacing behaviors. Included are quantitative analyses in seminatural environments which encourage a full range of social behaviors by the female, in their natural form. We have already discovered Direct Approaches, Head Turns, Hop-and-Stops, and Dart/Return Pacing responses. This is almost finished and will not take long. (Aim I1.): We will determine their hormonal facilitation. For the first time in mice, the relative importance of estrogens (E), progestins (P) and their combination will be explored. New data comparing the roles of P and the gene for PRs in small shoebox cages compared to a seminatural environment already show us the importance of context in discerning hormone/gene/behavior causal relations. (Aim III): We will discover their CNS sites of hormone action and genetic influences. For the genetics, primarily, a novel antisense technology will be used. Both hormonal experiments and antisense DNA experiments will include the use of a new 'microsphere' delivery system for hormones and for antisense DNA reagents. The antisense and microsphere techniques, well documented elsewhere, can be applied for the first time to the mouse CNS. Using female mice allows us to apply novel genetic tools. Analyzing the entire sequence of behaviors preceding lordosis, we can address scientifically exciting questions about reproductive behaviors which are not simple and which are biologically important. These behaviors bring together, at the right time, conspecifics who are competent to reproduce. Further, increasing attention to broader, hormone-dependent behavioral dispositions brings us one step closer to issues important for women's health.

在分析激素、神经和遗传机制方面取得了很大进展， 简单的性行为脊柱前凸现在，如果能理解导致人类行为的流程， 交配同时，探讨女性在这些感受性行为背后的行为倾向。 我们在雌性小鼠中发现了以前认为不存在的主动接近和踱步行为。 新的小鼠实验将允许“最先进的”遗传学阐明。 (Aim I.）：完成对这些运动、接近、接受和起搏行为的仔细描述。 包括在自然环境中的定量分析，鼓励全方位的社会 女性的自然行为我们已经发现了直接接近，头部转向， 跳跃和停止，和飞镖/返回起搏反应。这几乎完成了，不会花很长时间。 (Aim I1.）：我们将确定他们的荷尔蒙促进。首次在小鼠中， 雌激素（E）、孕激素（P）及其联合应用的研究。新数据比较P的作用 与自然环境相比，小鞋盒笼子里的PR基因已经向我们展示了 背景在辨别激素/基因/行为因果关系中的重要性。 (Aim我们将发现他们的中枢神经系统的激素作用和遗传影响。从遗传学角度来说， 首先将使用一种新的反义技术。激素实验和反义DNA 实验将包括使用一种新的激素和反义DNA的“微球”输送系统 试剂反义和微球技术，在其他地方有很好的记录，可以应用于 第一次进入小鼠中枢神经系统。 使用雌性小鼠使我们能够应用新的遗传工具。分析整个行为序列 在脊柱前凸之前，我们可以解决有关生殖行为的科学问题， 不简单，而且在生物学上很重要。这些行为在适当的时候， 有繁殖能力的同种物种。此外，人们越来越关注更广泛的激素依赖性 行为倾向使我们更接近对妇女健康重要的问题。