Muscle Regrowth during Physical Rehabilitation and Amino Acid Supplementation

描述（由申请人提供）：本提案的目的是进一步候选人？的科学发展的转化和临床研究，使他有可能过渡到成为一个独立的翻译科学家在康复。俄勒冈州大学提供了一个优秀的学术和研究环境和基础设施，以促进候选人？翻译研究的教育和培训。 职业目标：该奖项将支持候选人？通过提供一系列正式和非正式的职业发展活动，以及在一个特殊的指导团队的监督下进行动手学习和获取必要的初步数据的研究项目，成功地从初级研究员过渡到中级独立调查员。具体的短期职业目标将包括肌肉代谢，稳定同位素技术和康复研究的培训;负责任地进行研究和其他标准科学技能的培训;为获得实用的临床研究技能和康复科学未来研究应用的初步数据进行研究。长期的科学目标是使候选人成为骨骼肌代谢和康复方面的专家，并持续资助医学研究。 研究计划：一般的假设是，在老年人肌肉再生后，急性肌肉骨骼应力将受到积极的影响，传统的物理康复，并进一步加强营养补充。 利用最先进的稳定同位素方法学研究肌肉代谢和测量细胞信号传导的方法学，我们将检验以下具体假设：1a）确定TKR手术中缺血-再灌注损伤对骨骼肌细胞信号传导和基因表达的影响; 1b）确定肌细胞信号传导和基因表达的急性变化是否与2周后通过MRI测量的肌肉萎缩相关; 2）确定TKA后摄入EAA是否会急剧增加肌肉蛋白质合成; 3）确定PT康复期间补充EAA对TKA前后肌肉质量、力量和功能的影响。 公益：这项研究将把康复工作的重点放在具体的和目前尚未解决的机制，负责在老年人全膝关节置换术后的肌肉损失。虽然膝关节置换术后骨关节炎引起的膝关节疼痛通常会减轻，但很难完全恢复身体功能和独立性。这项研究将有助于恢复快速增长的膝关节炎老年人的身体功能和独立性。

DESCRIPTION (provided by applicant): The purpose of this proposal is to further the candidate?s scientific development in translational and clinical research and to make possible his transition to become an independent translational scientist in rehabilitation. The University of Oregon provides an outstanding academic and research environment and infrastructure to facilitate the candidate?s education and training in the conduct of translational research. Career Goals: This award will support the candidate?s successful transition from a junior researcher into a mid level independent investigator by providing an array of formal and informal career development activities, and a research project for hands-on learning and acquisition of essential preliminary data under the supervision of an exceptional mentoring team. The specific short-term career goals will include training in muscle metabolism, stable isotope techniques and rehabilitation research; training in the responsible conduct of research and other standard scientific skills; performance of a research study for the acquirement of practical clinical research skills and of preliminary data for future research applications in rehabilitation science. The long-term scientific goal is for the candidate to become an expert in skeletal muscle metabolism and rehabilitation with continuous funding for medical research. Research Plan: The general hypothesis is that in older adults muscle regrowth after an acute musculoskeletal stress will be positively influenced by traditional physical rehabilitation, and further enhanced by nutritional supplementation. Using state-of-the-art stable isotope methodologies for the study of muscle metabolism and methodologies for the measurement of cell signaling, we will test the following specific hypotheses: 1a) To determine the effects of ischemia-reperfusion injury on cell signaling and gene expression in skeletal muscle during TKR surgery; 1b) To determine if acute changes in muscle cell signaling and gene expression correlate with muscle atrophy measured 2 weeks later by MRI; 2) To determine if muscle protein synthesis is acutely increased with the ingestion of EAA following TKA; 3) To determine the effects of EAA Supplementation during PT rehabilitation on muscle mass, strength, and function before and after TKA. Public Benefit: This research will focus rehabilitation efforts on specific and currently unresolved mechanisms responsible for muscle loss following total knee replacement in older adults. While knee pain due to bone arthritis is often alleviated after knee replacement, complete return of physical function and independence is difficult to achieve. This research will help to restore physical function and independence in the rapidly growing population of older adults with knee arthritis.