PR COMPREHENSIVE CTR FOR HIV DISPARITIES: PSM: LAB CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 具体目标： 核心实验室活动的具体目标几乎保持不变。此外，该实验室提交了一份提案，希望成为新的RCMI临床和转化研究（RCTR）计划下的实验室核心的一部分，该计划通过波多黎各大学医学院（圣胡安）、加勒比中央大学（UCC; Bayamon）和庞塞医学院（PSM，Ponce）的联盟获得支持。该实验室核心将提供几乎相同类型的HIV相关检测，用于对参加该中心积极资助或新兴项目的患者进行临床评估。教育用户了解所提供测试的效用将是新增加的优先领域之一。以下测试通过核心实现。 1. HIV和HCV ELISA和HIV蛋白质印迹分析。 2. CD 4 T细胞计数的流式细胞术分析，T细胞亚群的细胞内细胞因子产生和细胞外产生的细胞因子-趋化因子的定量。 3.通过Roche Amplicor程序评估HIV和HCV病毒载量。 4. HCV基因分型（利帕）。 5.通过Siemens TruGene和内部程序进行HIV耐药突变分析（基因分型）。自2008年以来，这两种程序每两年由VQA进行一次熟练度测试，并获得世卫组织HIV ResNet的认证。 6. HIV辅助受体需求分析（CCR 5与CXCR 4）。 7. HIV感染的T细胞的流式细胞术分析。 8.人乳头瘤病毒（HPV）基因分型由罗氏HPV DNA阵列。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. SPECIFIC AIMS: The specific aims of the core laboratory activity remain virtually unchanged. In addition, the laboratory submitted a proposal to be a part of laboratory core under the new RCMI Clinical and Translational Research (RCTR) program, support for which was applied through the consortium of the University of Puerto Rico Medical Sciences campus (San Juan), Universidad Central del Caribe (UCC; Bayamon) and Ponce School of Medicine (PSM, Ponce). This laboratory core will provide virtually the same types of HIV related testing for clinical evaluation of patients enrolled in the center's actively funded or emerging projects. Education of the user population on the utility of tests being provided will be one of the newly added priority areas. The following tests are implemented through the core. 1. HIV and HCV ELISA and HIV Western blot analyses. 2. Flow cytometric analysis of CD4 T cell enumeration, intracytoplastic cytokine production by T cell subsets and quantification of extra-cellularly produced cytokines-chemokines. 3. HIV and HCV viral load assessment by Roche Amplicor procedures. 4. HCV Genotyping by the line-probe assay (LIPA). 5. HIV drug resistance mutation analysis (genotyping) by the Siemens TruGene and in-house procedures. Both procedures have been biannually proficiency tested by VQA and accredited by the WHO HIV ResNet since 2008. 6. HIV coreceptor requirement analysis (CCR5 vs. CXCR4). 7. Flow cytometric analysis of HIV infected T cells. 8. Human papillomavirus (HPV) genotyping by the Roche HPV DNA Array.