PR COMPREHENSIVE CTR FOR HIV DISPARITIES: PSM: LAB CORE

HIV 差异的公关综合点击率：PSM：实验室核心

• 批准号: 7961266
• 负责人: Yasuhiro Yamamura
• 金额: $ 21.63万
• 依托单位: UNIVERSITY OF PUERTO RICO MED SCIENCES
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-24 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7961266
• 关键词: Area; Biological Assay; CCR5 gene; CD4 Positive T Lymphocytes; CXCR4 gene; Clinical; Computer Retrieval of Information on Scientific Projects Database; DNA; Education; Enrollment; Enzyme-Linked Immunosorbent Assay; FOLH1 gene; Funding; Genotype; Grant; HIV; HIV drug resistance; Hepatitis C virus; Housing; Human Papillomavirus; Institution; Laboratories; Medical; Mutation Analysis; Patients; Population; Procedures; Production; Puerto Rico; Research; Research Personnel; Resources; Science; Source; T-Lymphocyte; T-Lymphocyte Subsets; Testing; Translational Research; United States National Institutes of Health; Universities; Viral Load result; Western Blotting; chemokine; cytokine; medical schools; programs; research clinical testing; resistance mutation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. SPECIFIC AIMS: The specific aims of the core laboratory activity remain virtually unchanged. In addition, the laboratory submitted a proposal to be a part of laboratory core under the new RCMI Clinical and Translational Research (RCTR) program, support for which was applied through the consortium of the University of Puerto Rico Medical Sciences campus (San Juan), Universidad Central del Caribe (UCC; Bayamon) and Ponce School of Medicine (PSM, Ponce). This laboratory core will provide virtually the same types of HIV related testing for clinical evaluation of patients enrolled in the center's actively funded or emerging projects. Education of the user population on the utility of tests being provided will be one of the newly added priority areas. The following tests are implemented through the core. 1. HIV and HCV ELISA and HIV Western blot analyses. 2. Flow cytometric analysis of CD4 T cell enumeration, intracytoplastic cytokine production by T cell subsets and quantification of extra-cellularly produced cytokines-chemokines. 3. HIV and HCV viral load assessment by Roche Amplicor procedures. 4. HCV Genotyping by the line-probe assay (LIPA). 5. HIV drug resistance mutation analysis (genotyping) by the Siemens TruGene and in-house procedures. Both procedures have been biannually proficiency tested by VQA and accredited by the WHO HIV ResNet since 2008. 6. HIV coreceptor requirement analysis (CCR5 vs. CXCR4). 7. Flow cytometric analysis of HIV infected T cells. 8. Human papillomavirus (HPV) genotyping by the Roche HPV DNA Array.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 具体目标： 核心实验室活动的具体目标几乎保持不变。此外，该实验室还提交了一份提案，希望成为新的 RCMI 临床和转化研究 (RCTR) 计划下实验室核心的一部分，该计划的支持是通过波多黎各大学医学科学校区（圣胡安）、加勒比中央大学（UCC；巴亚蒙）和庞塞医学院（PSM，庞塞）联合体申请的。该实验室核心将为参加该中心积极资助或新兴项目的患者提供几乎相同类型的艾滋病毒相关检测，以进行临床评估。对用户群体进行有关所提供测试实用性的教育将是新增的优先领域之一。下面的测试都是通过核心来实现的。 1. HIV 和 HCV ELISA 以及 HIV Western blot 分析。 2. CD4 T 细胞计数、T 细胞亚群产生的胞质内细胞因子以及细胞外产生的细胞因子-趋化因子的定量的流式细胞术分析。 3. 通过 Roche Amplicor 程序进行 HIV 和 HCV 病毒载量评估。 4.通过线探针测定(LIPA)进行HCV基因分型。 5. 通过西门子 TruGene 和内部程序进行 HIV 耐药突变分析（基因分型）。自 2008 年以来，这两种程序均经过 VQA 每两年一次的能力测试，并获得 WHO HIV ResNet 的认可。 6. HIV 辅助受体需求分析（CCR5 与 CXCR4）。 7. HIV感染的T细胞的流式细胞术分析。 8. 通过 Roche HPV DNA Array 进行人乳头瘤病毒 (HPV) 基因分型。