Matrix Metalloproteinase Gene Variants and Cleft Lip and Palate

基质金属蛋白酶基因变异与唇腭裂

• 批准号: 7933281
• 负责人: Ariadne M Letra
• 金额: $ 2.69万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7933281
• 关键词: 6H,8H-3,4-dihydropyrimido(4,5-c)(1,2)oxazin-7-one; Accounting; Affect; Award; Biological; Biological Assay; Biological Testing; Birth; Brazil; Candidate Disease Gene; Cleaved cell; Cleft Palate; Cleft lip with or without cleft palate; Clinical; Commit; Complex; Congenital Abnormality; DNA; Data; Dental; Dental Schools; Development; Development Plans; Disease; Economic Burden; Environment; Etiology; Evaluation; Event; Extracellular Matrix; Face; Family; Family Study; Foundations; Funding; Genes; Genetic; Genetic Counseling; Genetic Polymorphism; Genetic Predisposition to Disease; Genetic Transcription; Genetic Variation; Genomics; Goals; Grant; Growth; Hereditary Disease; Homeostasis; Hospitals; Human; In Vitro; Incidence; Individual; Inhibition of Matrix Metalloproteinases Pathway; Institution; Intervention; Investigation; Knock-out; Knowledge; Lip structure; Live Birth; Matrix Metalloproteinases; Medical; Medicine; Mentors; Metalloproteinase Gene; Modeling; Molecular; Molecular Biology; Mus; Normal tissue morphology; Nutritional; Oral; Organism; Organogenesis; Palate; Pattern; Peptide Hydrolases; Phase; Phenotype; Play; Population; Positioning Attribute; Postdoctoral Fellow; Predisposition; Prevalence; Principal Investigator; Process; Promoter Regions; Publications; Rehabilitation therapy; Reporting; Research; Research Design; Research Personnel; Role; Saliva; Sampling; Scientist; Secondary Palate; Signal Pathway; Societies; Source; Structure; Testing; Time; Tissue Inhibitor of Metalloproteinases; Tissues; Training; Transcriptional Regulation; Universities; Variant; base; career; career development; cleft lip and palate; craniofacial; dental genetics; experience; genetic variant; high risk; human tissue; improved; innovation; interest; migration; oral cleft; palatal fusion; palatal shelves; palatogenesis; post-doctoral training; professor; programs; psychologic; research study; retinal rods; skills; statistics; trait

DESCRIPTION (provided by applicant): Candidate: Dr. Ariadne Letra is a Postdoctoral Associate fellow at the University of Pittsburgh School of Dental Medicine. Her main research interests are genetics of oral-facial clefts. Her short-term goals are to improve knowledge of the multifactorial etiology of this condition and perform genetic studies of complex oral traits. The candidate also wishes to gain expertise in advanced statistical genetics and molecular biology to develop biological functional assays. Her long-term goal is to become an independent scientist and occupy a tenure track position at an Institution. This proposal reflects the plan of the candidate to engage in mentored-research meanwhile building a strong foundation for career development. The research plan is innovative as it proposes to study the family of matrix metalloproteinase genes which have been shown to have important roles during craniofacial development and implicated in cleft lip/palate. The candidate has important preliminary data that further support such evidence. Mentors: Dr. Alexandre Vieira will be the primary mentor for this proposal. He is the principal investigator on a grant for the expansion of the cleft phenotype in genetic studies of oral clefts and has a lot of expertise in studies with craniofacial and dental genetic disorders, with an impressive record of important publications. Dr. Mary Marazita will be the co-mentor for this proposal. She is an expert in statistical genetics and an acknowledged and well-funded researcher. She has extensive experience with mentoring trainees. Environment: the University of Pittsburgh has a long-standing track record of providing postdoctoral fellows with exceptional research and career development training, and offers great support for a successful transition into independent researcher. The School of Dental Medicine is fully committed to providing the candidate with an assistant professor position following the mentored-phase of the award. Research plan: specific aims for the K99 phase include replicate previous findings and investigate polymorphisms in all matrix metalloproteinase genes in association with clefting meanwhile improving skills in research design and statistics. For the ROD phase, the candidate wishes to perform functional assays to test the biological effects of genetic variants. Career Development Plan: the candidate plans to use the research plan to track her own progress as a scientist. Commitment of the mentor team will provide the candidate with frequent evaluations regarding scientific improvement and progress in career training.

描述（由申请人提供）：候选人：Ariadne Letra博士是匹兹堡大学牙科医学院的博士后副研究员。她的主要研究兴趣是口面部裂缝的遗传学。她的短期目标是提高对这种疾病的多因素病因学的认识，并对复杂的口腔特征进行遗传研究。候选人还希望获得先进的统计遗传学和分子生物学的专业知识，以开发生物功能测定。她的长期目标是成为一名独立的科学家，并在一个机构占据终身职位。该提案反映了候选人计划从事指导研究，同时为职业发展奠定坚实的基础。该研究计划是创新的，因为它提出研究基质金属蛋白酶基因家族，这些基因已被证明在颅面发育过程中发挥重要作用，并与唇腭裂有关。候选人拥有重要的初步数据，进一步支持这些证据。导师：Alexandre Vieira博士将是本提案的主要导师。他是一项用于扩大唇腭裂遗传学研究中唇腭裂表型的拨款的首席研究员，在颅面和牙齿遗传疾病的研究方面拥有丰富的专业知识，并发表了令人印象深刻的重要出版物。玛丽·马拉齐塔博士将担任该提案的共同导师。她是一位统计遗传学专家，也是一位公认的、资金充足的研究人员。她在指导学员方面拥有丰富的经验。工作环境：匹兹堡大学在为博士后研究员提供卓越的研究和职业发展培训方面有着悠久的历史，并为他们成功过渡到独立研究人员提供了巨大的支持。牙科医学院完全致力于为候选人提供助理教授职位后，指导阶段的奖励。研究计划：K99阶段的具体目标包括重复以前的发现，并调查与裂相关的所有基质金属蛋白酶基因的多态性，同时提高研究设计和统计学方面的技能。对于ROD阶段，候选人希望进行功能测定，以测试遗传变异的生物学效应。职业发展计划：候选人计划使用研究计划来跟踪自己作为科学家的进展。导师团队的承诺将为候选人提供有关科学进步和职业培训进展的频繁评估。

项目成果

Studies with Wnt genes and nonsyndromic cleft lip and palate.

• DOI: 10.1002/bdra.20720
• 发表时间: 2010-11
• 期刊: BIRTH DEFECTS RESEARCH PART A-CLINICAL AND MOLECULAR TERATOLOGY
• 作者: Menezes, Renato;Letra, Ariadne;Kim, Ana H.;Kuechler, Erika C.;Day, Alicia;Tannure, Patricia N.;da Motta, Luise Gomes;Paiva, Katiucia B. S.;Granjeiro, Jose M.;Vieira, Alexandre R.
• 通讯作者: Vieira, Alexandre R.

Interleukin-8 gene promoter polymorphism (rs4073) may contribute to chronic periodontitis.

• DOI: 10.1902/jop.2010.100513
• 发表时间: 2011-06
• 期刊: Journal of periodontology
• 影响因子: 4.3
• 作者: Andia DC;de Oliveira NF;Letra AM;Nociti FH Jr;Line SR;de Souza AP
• 通讯作者: de Souza AP