Interleukin-8 gene promoter polymorphism (rs4073) may contribute to chronic periodontitis.
Interleukin-8 gene promoter polymorphism (rs4073) may contribute to chronic periodontitis.
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DOI：
10.1902/jop.2010.100513
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发表时间：
2011-06
影响因子：
4.3
通讯作者：
de Souza AP
中科院分区：
文献类型：
--
作者：
Andia DC;de Oliveira NF;Letra AM;Nociti FH Jr;Line SR;de Souza AP
促炎性趋化因子白介素8(IL8)在炎症反应的调节中起重要作用。单核苷酸多态性(SNP)rs4073研究表明，内毒素刺激后，A等位基因可上调IL-8水平。我们调查了SNP rs4073与慢性牙周炎的关系。采用标准聚合酶链式反应-限制性片段长度多态性分析方法对289例健康对照和慢性牙周炎患者的基因组DNA进行基因分型，并用多因素Logistic回归模型进行校正分析。采用实时荧光定量聚合酶链式反应(Real-Time-PCR)检测IL-8基因的表达水平。分析表明，TA杂合子与慢性牙周炎有统计学意义的相关性(p=0.001)；结果显示，A等位基因在疾病组中的频率增加(对照组为36%，牙周炎组为48%)。IL8基因在牙周炎组中的表达水平较高，主要表现在TA基因携带者(p=0.03)。在不吸烟的巴西受试者中，SNP rs4073与慢性牙周炎有关，因为A等位基因在疾病中的频率高于对照组，而TA基因型与IL8 mRNA转录水平的升高有关。
The proinflammatory chemokine interleukin-8 (IL8) is important in the regulation of the inflammatory response. Single nucleotide polymorphism (SNP) rs4073 have shown that the A allele up regulates the IL8 levels after stimulation with lipopolysaccharides. We investigated the association of the SNP rs4073 with chronic periodontitis. Genotyping was performed by standard polymerase chain reaction-restriction fragment length polymorphism assay in 289 genomic DNA samples of healthy control and chronic periodontitis patients; analyses were adjusted by the multivariate logistic regression modeling. Real-time PCR performance was used to detect the levels of the IL8 mRNA. Analysis points to a statistical significant association of chronic periodontitis with the heterozygous TA genotype (p = 0.001); the results showed an increase in the frequency of the A allele in the diseased group (36% in the control × 48% in the periodontitis group). The higher levels of the IL8 mRNA were found in the periodontitis group, mainly in individuals who presented the TA genotype (p=0.03). The SNP rs4073 is associated with chronic periodontitis, in non-smoker Brazilian subjects, since the frequency of A allele is higher in the disease than control group and the TA genotype was associated with increased levels of IL8 mRNA transcripts.
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影响因子：
3.8
作者：
Garza-Gonzalez E;Bosques-Padilla FJ;Mendoza-Ibarra SI;Flores-Gutierrez JP;Maldonado-Garza HJ;Perez-Perez GI
通讯作者：
Perez-Perez GI
影响因子：
6.7
作者：
Astolfi, Claudia Maria;Shinohara, Andre Luis;de Souza, Ana Paula
通讯作者：
de Souza, Ana Paula
DOI：
10.1136/bjo.2007.123190
发表时间：
2008-04
期刊：
The British journal of ophthalmology
影响因子：
--
作者：
Goverdhan SV;Ennis S;Hannan SR;Madhusudhana KC;Cree AJ;Luff AJ;Lotery AJ
通讯作者：
Lotery AJ
影响因子：
18.6
作者：
Page, RC;Offenbacher, S;Kornman, KS
通讯作者：
Kornman, KS
影响因子：
6.7
作者：
Kornman, KS;Crane, A;Duff, GW
通讯作者：
Duff, GW