Gene Transfer Vector Core

基因转移载体核心

• 批准号: 7900760
• 负责人: Beverly L. Davidson
• 金额: $ 12.13万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7900760
• 关键词: Adenovirus Vector; Adenoviruses; Automobile Driving; Cancer Center Support Grant; Cell Line; Clinical; Complementary DNA; Consultations; Dependovirus; Development; Discipline; Doctor of Medicine; Doctor of Philosophy; Feline Immunodeficiency Virus; Fertilization; Gene Expression; Gene Transfer; Generations; Histocompatibility Testing; Iowa; Methods; Oncogenes; Other Multiple Areas; Phase; Plasmids; Preparation; Principal Investigator; Recombinants; Reporter; Reporter Genes; Research; Research Personnel; Retroviridae; Services; Shuttle Vectors; Subfamily lentivirinae; Technical Expertise; Technology; Testing; United States National Institutes of Health; Universities; Virus; cost; design; experience; gene transfer vector; innovation; interest; novel; promoter; recombinant virus; research and development; success; tool; vector

Gene transfer is becoming increasingly important as a research tool and has clinical potential as well. The Gene Transfer Vector Core (GTVC) brings expertise to cancer from gene transfer experience in multiple other areas of biomedicine. The interaction with multiple investigators from various disciplines and experience with multiple different types of tissues allows for cross-fertilization of ideas, technical advancements, and innovations in vector technologies. The GTVC provides 1) Consultation with the Principal Investigator 2) Development of novel vectors 3) Purified and concentrated preparations of recombinant adenovirus, adeno-associated virus (AAV), and retrovirus (including lentivirus) 4) Collaborative testing of vectors generated for function and purity, and finally routine preparation 5) Standard cell lines, expression plasmids, and stocks of recombinant reporter viruses GTVC staff and investigators are in close contact through all phases of vector design and generation. Thus, the GTVC serves as both a research and development facility for gene transfer studies, and a service facility for routine vector preparations

基因转移作为一种研究工具正变得越来越重要，并且也具有临床潜力。 基因转移载体核心（GTVC）从基因转移经验中为癌症带来专业知识， 生物医学的多个其他领域。与来自不同领域的多名研究人员的互动 学科和经验与多种不同类型的组织允许交叉施肥的想法， 技术进步和载体技术的创新。GTVC提供 1)与主要研究者协商 2)新型载体的开发 3)重组腺病毒、腺相关病毒的纯化和浓缩制剂 (AAV)和逆转录病毒（包括慢病毒） 4)对生成的载体进行功能和纯度的协作检测，最后进行常规制备 5)重组报告病毒的标准细胞系、表达质粒和储备液 GTVC工作人员和调查人员在载体设计和生成的所有阶段都保持密切联系。 因此，GTVC既是基因转移研究的研究和开发设施， 常规病媒制剂服务设施