PATHOGENESIS OF AN X4-TROPIC VARIANT OF SIVMAC239
PATHOGENESIS OF AN X4-TROPIC VARIANT OF SIVMAC239
批准号:
7958703
负责人:
James A Hoxie
金额:
$5.81万
依托单位国家:
美国
项目类别:
财政年份:
2009
资助国家:
美国
项目状态:
已结题
起止时间:
2009-05-01 至 2010-04-30
关键词:
Acquired Immunodeficiency SyndromeAnimalsBiopsyBlood specimenCCR5 geneCD4 Positive T LymphocytesCXCR4 geneChemistryComputer Retrieval of Information on Scientific Projects DatabaseExhibitsFlow CytometryFundingGrantHealthImmune responseIn SituIn VitroInfectionInstitutionIntestinesLeukocytesMacaca mulattaMeasuresMonitorPathogenesisPlasmaPrimatesProtocols documentationResearchResearch PersonnelResourcesSourceTestingUnited States National Institutes of HealthVariantViralViral Load resultVirusWhole Bloodin vivolymph nodesmalereceptor
中文摘要
这个子项目是众多研究子项目之一
英文摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
We have derived, in vitro, a variant of SIVmac239, termed SIVmac239-ST1, that exhibits high efficiency use of X4. We used this new virus to infect four male rhesus macaques to assess the effects of its altered co-receptor usage on in vivo viral replication and host control. In this protocol we intravenously inoculated four Rhesus macaques of Indian origin with 1X103 TCID50 of SIVmac239-ST1. Blood samples were collected for flow cytometry analysis as well as to monitor plasma viral load, humoral immune responses and the overall health of the animals (CBC and Chemistry). CSF was also collected for viral load analysis. Lymph node and intestinal biopsies were used for localization of the virus in situ as well as isolation of leukocytes and flow cytometry analysis. Physical exams were performed to monitor the overall health of the animals. All four animals became infected and had peak viral loads between 6.5 and 7.2 log copies/ml measured by bDNA. The set point of infection ranged between 3.5 and 6.5 log copies/ml. One animal became sick and was humanely euthanized 368 days post-challenge. The other three animals were humanely euthanized on 314 days (n=2) and 371 days (n=1) post-challenge. CCR5 decreased dramatically after infection in all four animals and in the lymph node of one animal. CXCR4 also decreased in the gut of two animals after infection (the other two were not tested). CD4 decreased in the gut of all four animals after infection. CD4 cells steadily declined in the whole blood of the one animal that showed signs of AIDS.
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财政年份:2014
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负责人:James A Hoxie
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依托单位:
CONTROL OF AN SIVMAC239 TRANSMEMBRANE MUTANT IN PIGTAIL MACAQUES
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资助金额:$5.78万
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财政年份:2011
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负责人:James A Hoxie
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依托单位:
PATHOGENESIS OF AN ATTENUATED SIVMAC239 IN RHESUS AND PIGTAIL MACAQUES
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批准号:8358095
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资助金额:$5.78万
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财政年份:2011
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负责人:James A Hoxie
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依托单位:
Optimizing HIV Env immuogens for T and B cell vaccine responses
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资助金额:$51.77万
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财政年份:2010
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依托单位:
Optimizing HIV Env immuogens for T and B cell vaccine responses
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批准号:8091276
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项目类别:
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资助金额:$65.41万
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财政年份:2010
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负责人:James A Hoxie
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依托单位:
Optimizing HIV Env immuogens for T and B cell vaccine responses
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批准号:7988637
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项目类别:
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资助金额:$58.7万
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财政年份:2010
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负责人:James A Hoxie
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依托单位:
Optimizing HIV Env immuogens for T and B cell vaccine responses
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批准号:8300199
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项目类别:
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资助金额:$55.19万
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财政年份:2010
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负责人:James A Hoxie
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依托单位:
PATHOGENIC DETERMINANTS OF THE SIV ENVELOPE TRANSMEMBRANE CYTOPLASMIC DOMAIN
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批准号:8173001
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项目类别:
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资助金额:$6.18万
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财政年份:2010
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负责人:James A Hoxie
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依托单位:
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项目类别:
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资助金额:$6.27万
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负责人:James A Hoxie
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依托单位:
Pilot Projects
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批准号:7981648
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项目类别:
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资助金额:$24.67万
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财政年份:2009
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负责人:James A Hoxie
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依托单位:
Administrative
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批准号:7684965
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资助金额:$29.47万
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依托单位:
Pathogenic determinants of the SIV envelope transmembrane cytoplasmic domain
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批准号:8471046
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资助金额:$95.15万
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财政年份:2008
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依托单位:
Pathogenic determinants of the SIV envelope transmembrane cytoplasmic domain
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资助金额:$79.52万
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依托单位:
Pathogenic determinants of the SIV envelope transmembrane cytoplasmic domain
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批准号:7579899
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资助金额:$94.16万
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财政年份:2008
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依托单位:
Pathogenic determinants of the SIV envelope transmembrane cytoplasmic domain
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批准号:8279208
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项目类别:
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资助金额:$81.37万
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财政年份:2008
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负责人:James A Hoxie
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依托单位:
Administrative Core
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项目类别:
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资助金额:$25.96万
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财政年份:2008
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Pathogenic determinants of the SIV envelope transmembrane cytoplasmic domain
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财政年份:2008
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负责人:James A Hoxie
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依托单位:
海外基金