Differential fertilisation compatibility in Atlantic salmon: implications for farmed salmon gene introgression and hybridisation

大西洋鲑鱼的差异受精兼容性：对养殖鲑鱼基因渗入和杂交的影响

• 批准号: NE/E007279/1
• 负责人: Matthew Gage
• 金额: $ 42.15万
• 依托单位: University of East Anglia
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=NE%2FE007279%2F1
• 关键词: Differential; fertilisation; compatibility; Atlantic; salmon

One of the most important barriers to reproduction between different species is compatibility between sperm and egg. Biologists are trying to understand the evolution of recognition and compatibility between gametes, but for most species nothing is known about how sperm and egg co-evolve to recognise one-another for fertilization. Recent research shows that this process probably evolves very quickly, probably because of sperm competition between males to fertilise the eggs, and conflict between males and females to get the most out of reproduction. We will examine the compatibility between sperm and egg in Atlantic salmon, which is an ideal model to examine these questions for both pure and applied reasons. Salmon adults return to the river where they hatched to reproduce. This strategy means that genetic mixing between different populations is low, and biologists have shown that different strains have clear genetic differences, and have evolved specific adaptations for their own population (such as size, spawning age etc). These isolating conditions make salmon a likely species to have evolved differences between populations in sperm and egg compatibility, because of those rapid evolutionary processes just mentioned. We have found evidence for differences in fertilization compatibility between salmon strains, based on quite specific, but important immunity genes. In this project we plan to examine these differences in much more breadth by looking at how sperm and egg fertilization compatibility has changed between different populations, while also applying these theories to a key problem facing wild Atlantic salmon. Wild Atlantic salmon are an important fish for many reasons, but are unfortunately in severe decline. A major problem facing wild salmon stocks is the escape of farmed salmon, which occurs over the salmon's range at a very high level. Escaped fish can survive, and some make it onto wild salmon spawning grounds. Here they attempt to spawn with wild fish, presenting a major problem: farmed salmon have been domestically-selected for big genetic differences with wild fish. Offspring from farmed and wild salmon reproduction carry inferior farmed genes, and mean that important wild genes in different populations become diluted. Continual release of so many farmed fish could eventually genetically swamp wild genes, possibly permanently losing specifically evolved local adaptations forever. One could imagine a similar situation if domestic dogs were continually released into wolf populations to breed; eventually the pure, wild wolf genes would be lost if release occurred every year at high enough rates. A vital piece of information in understanding the level of the farmed salmon problem is how fertile are crosses between farmed and wild salmon? Domestication might have altered farmed salmon fertility, either up or down. Research has shown that farmed fish can successfully reproduce with wild fish, but that they are inferior at reproduction. However, exactly HOW inferior are farmed fish, and how does this inferiority vary when reproducing with different wild populations? This information is important to understand the risk of farmed fish to wild stocks. We will answer this question by measuring the fertility of farmed sperm and eggs with wild salmon sperm and eggs. We have developed trials that run fertilisations in controlled conditions, but which mimic the normal function of sperm and egg in a salmon river. We will also examine fertilisation success when wild and farmed sperm mix and compete for a batch of eggs, a process which occurs normally in the wild. We will also measure how fertile farmed salmon are with a close relative, trout, since this is another possible way for farmed hybrids to be produced. Our research will allow us to measure the relative fertility of farmed salmon, thus providing very useful information for governments balancing wild fish conservation with commercially important salmon farming.

不同物种之间繁殖的最重要障碍之一是精子和卵子之间的相容性。生物学家正试图了解配子之间的识别和兼容性的进化，但对于大多数物种来说，对于精子和卵子如何共同进化以识别彼此进行受精一无所知。最近的研究表明，这一过程可能进化得非常快，可能是因为雄性之间的精子竞争使卵子受精，以及雄性和雌性之间的冲突，以获得最大的繁殖。我们将研究大西洋鲑鱼精子和卵子之间的相容性，这是一个理想的模型来研究这些问题的纯粹和应用的原因。成年鲑鱼回到它们孵化的河流中繁殖。这种策略意味着不同种群之间的遗传混合很低，生物学家已经证明，不同的菌株具有明显的遗传差异，并为自己的种群（如大小，产卵年龄等）进化出特定的适应性。这些孤立的条件使鲑鱼成为一个可能在精子和卵子相容性方面进化出种群差异的物种，因为这些快速的进化过程刚刚提到。我们已经发现了鲑鱼品系之间受精相容性差异的证据，这是基于非常特定但重要的免疫基因。在这个项目中，我们计划通过研究不同种群之间精子和卵子受精的相容性如何变化来更广泛地研究这些差异，同时将这些理论应用于野生大西洋鲑鱼面临的一个关键问题。野生大西洋鲑鱼是一种重要的鱼类，原因有很多，但不幸的是，它们的数量正在严重下降。野生鲑鱼种群面临的一个主要问题是养殖鲑鱼的逃逸，这在鲑鱼的范围内发生在一个非常高的水平。逃脱的鱼可以生存，有些使野生鲑鱼产卵场。在这里，他们试图与野生鱼类一起产卵，这带来了一个主要问题：养殖鲑鱼已经被驯化，因为它们与野生鱼类有很大的遗传差异。养殖和野生鲑鱼繁殖的后代携带劣质养殖基因，这意味着不同种群中重要的野生基因被稀释。如此多的养殖鱼类的持续释放最终可能会在遗传上淹没野生基因，可能会永远失去特定的进化适应性。我们可以想象，如果家犬被不断地释放到狼的种群中繁殖，也会出现类似的情况;如果每年都以足够高的速度释放，那么最终纯野生狼的基因就会丢失。了解养殖鲑鱼问题的程度的一个重要信息是养殖鲑鱼和野生鲑鱼之间的杂交有多肥沃？驯化可能改变了养殖鲑鱼的繁殖力，无论是上升还是下降。研究表明，人工养殖的鱼类可以成功地与野生鱼类繁殖，但它们的繁殖能力较差。然而，养殖鱼到底有多差，当与不同的野生种群繁殖时，这种差又有何不同？这一信息对于了解养殖鱼类对野生种群的风险很重要。我们将通过测量养殖的精子和卵子与野生鲑鱼精子和卵子的生育能力来回答这个问题。我们已经开发了在受控条件下进行受精的试验，但这模拟了鲑鱼河中精子和卵子的正常功能。我们还将研究野生和养殖精子混合并竞争一批卵子时的受精成功率，这一过程通常发生在野外。我们还将测量养殖鲑鱼与近亲鳟鱼的繁殖力，因为这是养殖杂交种的另一种可能的生产方式。我们的研究将使我们能够测量养殖鲑鱼的相对繁殖力，从而为政府平衡野生鱼类保护与商业上重要的鲑鱼养殖提供非常有用的信息。

项目成果

Rapid rates of sperm DNA damage after activation in tench (Tinca tinca: Teleostei, Cyprinidae) measured using a sperm chromatin dispersion test.

使用精子染色质分散测试测量丁丁鱼（Tincatinca：Teleostei，Cyprinidae）激活后精子 DNA 快速损伤率。

• DOI: 10.1530/rep-09-0105
• 发表时间: 2009
• 期刊: Reproduction (Cambridge, England)
• 作者: López-Fernández C

Assessing risks of invasion through gamete performance: farm Atlantic salmon sperm and eggs show equivalence in function, fertility, compatibility and competitiveness to wild Atlantic salmon.

• DOI: 10.1111/eva.12148
• 发表时间: 2014-04
• 期刊: Evolutionary applications
• 影响因子: 4.1
• 作者: Yeates SE;Einum S;Fleming IA;Holt WV;Gage MJ
• 通讯作者: Gage MJ

Cryptic choice of conspecific sperm controlled by the impact of ovarian fluid on sperm swimming behavior.

• DOI: 10.1111/evo.12208
• 发表时间: 2013-12
• 期刊: Evolution; international journal of organic evolution
• 作者: Yeates SE;Diamond SE;Einum S;Emerson BC;Holt WV;Gage MJ
• 通讯作者: Gage MJ

Post-copulatory opportunities for sperm competition and cryptic female choice provide no offspring fitness benefits in externally fertilizing salmon.

• DOI: 10.1098/rsos.150709
• 发表时间: 2016-03
• 期刊: Royal Society open science
• 影响因子: 3.5
• 作者: Lumley AJ;Diamond SE;Einum S;Yeates SE;Peruffo D;Emerson BC;Gage MJ
• 通讯作者: Gage MJ