IN VITRO STUDIES OF HIV IN PRIMARY CULTURES OF HUMAN BLOOD CELLS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 研究Nef对人类细胞原代培养物中蛋白质运输和病毒感染性的影响。 Nef是HIV-1毒力的重要贡献者。已经研究了与连续人类细胞系中NEF表达相关的多种表型，包括其下调来自细胞表面的CD 4和I类MHC以及优化病毒感染性的能力。 细胞转染方法的最新改进现在允许将这些研究扩展到最相关的细胞，原代人T淋巴细胞。 T细胞活化可能影响Nef的性质。该假设在非转化细胞中得到最好的检验。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Study the effects of Nef on protein trafficking and viral infectivity in primary cultures of human cells. Nef is a significant contributor to the virulence of HIV-1. A variety of phenotypes associated with NEF-expression in continuous human cells lines including its ability to down-regulate CD4 and class I MHC from the cell surface and to optimize viral infectivity have been studied. Recent improvements in cell-transfection methods now allow extension of these studies to the most relevant cells, primary human T lymphocytes. T cell activation may influence the properties of Nef. This hypothesis is best tested in non-transformed cells.