EVALUATING EFFECTS OF MARIJUANA SMOKING ABILITY TO RESPOND TO HEPATITIS B VACCIN

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 A. 具体目标 本临床研究的主要目的是确定吸入MJ烟雾和THC对抗原激发的人体免疫应答的影响，以及MJ使用增加严重病毒感染风险的可能性（通过对B型肝炎疫苗接种的免疫应答进行测量）。将确定以下具体成果： “ELISA测定法测定的抗人球蛋白抗体滴度。 T细胞增殖 通过流式细胞术分析确定的HBsAg特异性T细胞的频率和以下亚群的分布：CD 4/CD 8应答者，Th 1/Th 2/Treg细胞，以及幼稚T细胞与记忆T细胞。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A. SPECIFIC AIMS The primary objective of this clinical study is to determine the impact of inhaled MJ smoke and THC on human immune responses to an antigen challenge and the potential for MJ use to increase the risk for serious viral infections as measured by the immune response to Hepatitis B vaccination. The following specific outcomes will be determined: " HBsAb titer by ELISA assay. " HBsAg-specific T cell proliferation " Frequency of HBsAg-specific T cells and the distribution of the following subsets as determined by flow cytometry analysis: CD4/CD8 responders, Th1/Th2/Treg cells, and na¿ve versus memory T cells.