LDL

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 低密度脂蛋白转变为氧化型低密度脂蛋白，可被巨噬细胞迅速摄取，导致冠状动脉疾病。对位素酶是一种高密度脂蛋白结合的多态酶，可降低氧化低密度脂蛋白水平，从而预防冠心病。据推测，少量饮酒会增加血清PON水平，并通过阻止其形成或破坏它来降低OxLDL的水平，从而具有心脏保护作用，而大量饮酒80g/天则有相反的副作用。具体目标是： 1.测定轻度饮酒者和重度饮酒者PON的相对浓度和活性。 2.研究PON基因的遗传多态性及其与酒精暴露程度的相关性。 3.测定血浆OxLDL浓度，并探讨其与PON活性及冠心病发病的关系。 4.测定各组高密度脂蛋白S将生物活性氧化低密度脂蛋白转化为生物活性低密度脂蛋白的能力及与PON活性的关系。抗PON能否抑制高密度脂蛋白对氧化低密度脂蛋白的破坏作用&S 5.测定各组高密度脂蛋白S对巨噬细胞摄取氧化低密度脂蛋白的能力，并与相应的PON活性进行相关分析。确定抗PON在哪里能特异性阻断高密度脂蛋白S的保护作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. LDL to oxidized LDL (OxLDL) that is rapidly taken up by macrophages leading to coronary artery diseae (CAD). Paraxonase (PON), a HDL-bound polymorphic anzyme lowers OxLDL level and thus prevents CAD. It is hypothesized that light drinking (13-40g/day) increases serum PON level and theryby lowers OxLDL by preventing its formationor by destroying it and thus has a cardioprotective effect, whereas heavy drinking (>80g/day) has the opposite side effects. The specific aims are: 1. To determine the relative concentration and activiry of the PON in light and heavy drinkers. 2. To characterize the genetic polymorphism of PON gene and correlate with the extent of alcohol exposure. 3. To determine the concentration of plasm OxLDL and correlate with their PON activity and the incidence of CAD. 4. To determine the ability of HDL's from each group to convert the biologically active oxidized-LDL to biologically inactive form and corrlate with corresonding PON acitiviy. To determine whether anti-PON can inhibit the destruction of OxLDL by HDL's 5. To determine the ability of HDL's from each group to prvent the uptake of OxLDL by macrophages and correlate with the corresponding PON activity. To determin where Anti-PON can specifically block the protective effecgt of HDL's.