SGC TORONTO: CANADIAN MEDICAL PROTEIN-STRUCTURE HUNT

SGC 多伦多：加拿大医学蛋白质结构搜寻

• 批准号: 7955548
• 负责人: Alexei Bochkarev
• 金额: $ 2.02万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955548
• 关键词: British; Computer Retrieval of Information on Scientific Projects Database; Data; Funding; Goals; Grant; Hereditary Disease; Home environment; Human; Human Genetics; Institution; Medical; Metabolism; Methods; Molecular; Proteins; Research; Research Personnel; Resolution; Resources; Robot; Scientist; Source; Structure; United States National Institutes of Health; beamline; protein degradation; protein structure; small molecule; structural genomics; synchrotron radiation; therapeutic protein; three dimensional structure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Structural Genomics Consortium (SGC) is a not-for-profit British-Canadian partnership that aims to determine the 3-dimentional structures of hundreds of proteins of significant medical importance. The SGC Toronto scientists seek to determine the 3D structures of human proteins of therapeutic relevance, with a probable focus on those involved in intracellular small molecule metabolism, those with a direct involvement in human genetic disease and those that are implicated in protein degradation. It is likely that most of the structures at the SGC will be determined using the method of molecular replacement. We expect >70% of the SGC crystal structures will be determined using data collected at home with Rigaku FR-E and with an Actor robot for crystal mounting. Synchrotron radiation will be necessary to collect higer resolution data for poor diffracting crystals. Goal of this proposal is to evaluate the resolution increase for data collected with CHESS beamline A1 and F1 over that with Rigaku FR-E (at home).

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 结构基因组学联盟(SGC)是一个非营利性的英国和加拿大合作伙伴关系，旨在确定数百种具有重大医学意义的蛋白质的三维结构。 多伦多SGC的科学家试图确定与治疗相关的人类蛋白质的3D结构，可能重点放在那些参与细胞内小分子代谢的蛋白质、那些直接参与人类遗传病的蛋白质以及那些与蛋白质降解有关的蛋白质。很可能SGC的大部分结构将使用分子置换的方法来确定。 我们预计70%的SGC晶体结构将使用在家中使用Rigaku FR-E和Actor机器人进行晶体安装所收集的数据来确定。为了收集低衍射晶体的高分辨率数据，同步辐射将是必要的。 这项建议的目标是评估使用国际象棋光束线A1和F1收集的数据的分辨率比使用Rigaku FR-E(在家)收集的数据的分辨率提高。