SGC TORONTO: CANADIAN MEDICAL PROTEIN-STRUCTURE HUNT

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 结构基因组学联盟（SGC）是一个非营利性的英国-加拿大合作伙伴关系，旨在确定数百种具有重要医学意义的蛋白质的三维结构。 SGC多伦多的科学家试图确定具有治疗相关性的人类蛋白质的3D结构，可能重点关注那些参与细胞内小分子代谢的蛋白质，那些直接参与人类遗传疾病的蛋白质以及那些与蛋白质降解有关的蛋白质。这是可能的，在SGC的大部分结构将使用分子置换的方法来确定。 我们预计>70%的SGC晶体结构将使用理学FR-E和Actor机器人在家收集的数据进行晶体安装。同步辐射将是必要的，以收集更高的分辨率数据差衍射晶体。 本提案的目的是评估CHESS光束线A1和F1与理学FR-E（在国内）相比所收集数据的分辨率增加。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Structural Genomics Consortium (SGC) is a not-for-profit British-Canadian partnership that aims to determine the 3-dimentional structures of hundreds of proteins of significant medical importance. The SGC Toronto scientists seek to determine the 3D structures of human proteins of therapeutic relevance, with a probable focus on those involved in intracellular small molecule metabolism, those with a direct involvement in human genetic disease and those that are implicated in protein degradation. It is likely that most of the structures at the SGC will be determined using the method of molecular replacement. We expect >70% of the SGC crystal structures will be determined using data collected at home with Rigaku FR-E and with an Actor robot for crystal mounting. Synchrotron radiation will be necessary to collect higer resolution data for poor diffracting crystals. Goal of this proposal is to evaluate the resolution increase for data collected with CHESS beamline A1 and F1 over that with Rigaku FR-E (at home).