HIGH RESOLUTION DATA SET OF 2 FORMS OF VANCOMYCIN RESISTANCE ACCOCIATED

两种形式的万古霉素耐药性的高分辨率数据集

• 批准号: 7955562
• 负责人: EMIL PAI
• 金额: $ 1.99万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955562
• 关键词: Active Sites; Anabolism; Anodes; Archaea; Bacteria; Binding; Cell Wall; Cleaved cell; Computer Retrieval of Information on Scientific Projects Database; Consensus; Data; Data Set; Enzymes; Escherichia coli; Freezing; Funding; Grant; Institution; Mammals; Peptides; Peptidoglycan; Pharmaceutical Preparations; Proteins; Research; Research Personnel; Resolution; Resources; Serine Protease; Source; Structure; Temperature; Testing; Time; United States National Institutes of Health; Vancomycin; Vancomycin Resistance; Work; inorganic phosphate; mutant; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. 1) Vancomycin resistance accociated Regulator (VraR) is involved in mounting a response to antibiotices that target cell wall peptidoglycan biosynthesis such as vancomycin. This enzyme is not present in mammals and thus becomes a good target for a drug to target vancomycin resistant bacteria. (2 MAD data sets + 2 high resolution) (1 MAD set and high res is absolutely needed now, the highest priority) The other MAD set and high res is the second thing that we need from this trip. Also, 2 high resolution sets of a different space group would be collected last on the trip. Only if there is extra time, this is not a primary data set. All of these crystals have been frozen and pre-screened on our rotating anode --------------------------------------------------------------- 2) E. coli serine protease (ECSP) cleaves several significant proteins. We have previously solved the structure and would now like to look at consensus peptides bound to the active site. 2 data sets - The 2.5A was low exposure just to check the crystal quality of pre-frzen crystals. Crystals are ordered to better than 2A. All of these crystals have been frozen and pre-screened on our rotating anode. These will diffract to higher resolution at CHESS. The native protein diffracts much better, better than 2A. We are trying to get some additional similar standard peptides into the protein. If these work before the trip, we will pre-freeze and pre-screen them and bring them along to collect. -------------------------------------------------------------- 3) (S)-3-O-Geranylgeranlyglyceryl Phosphate Synthase (GGGPS) is isolated from Archaea and is involved in synthesis of compounds unique to Archaea cell walls. We would like to look at the mechanism of this unique type of enzymes. 3 data sets - they have been frozen and pre-screened on our rotating anode and stored for the trip. There are a few other similar mutants being crystallized. If we can get crystals, pre-freeze and pre-test them, we will bring them along to collect, if there is time. ------------------------------------------------------------------ Every crystal on our list has been pre-frozen and pre-screened on our rotating anode. We will bring some room temperature plates, but purely as a backup. The MAD data on VraR is our priority for this trip.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 1)万古霉素耐药相关调节因子（VraR） 对靶向细胞壁肽聚糖生物合成的抗生素如万古霉素产生应答。 这种酶不存在于哺乳动物中，因此成为药物靶向万古霉素耐药细菌的良好靶标。 （2个MAD数据集+ 2个高分辨率）（1个MAD数据集和高分辨率是现在绝对需要的，最高优先级）另一个MAD数据集和高分辨率是我们这次旅行需要的第二件事。 此外，在旅行的最后一站将收集到不同空间群的2个高分辨率集。 只有在有额外时间的情况下，这才不是主要数据集。 所有这些晶体都被冷冻并在我们的 旋转阳极 --------------------------------------------------------------- 2)E.大肠杆菌丝氨酸蛋白酶（ECSP）切割几种重要的蛋白质。 我们以前已经解决了结构，现在将 我想看看结合在活性位点上的共有肽。 2个数据集-2.5A为低曝光，仅用于检查预熔晶体的晶体质量。 晶体有序度优于2A。所有这些晶体都被冷冻并在我们的旋转阳极上预先筛选。 这些将在国际象棋中实现更高的分辨率。 天然蛋白质衍射好得多，比2A好。 我们正试图在蛋白质中加入一些额外的类似标准肽。 如果这些工作之前的旅行，我们将预先冻结和预先筛选他们，并把他们一起收集沿着。 -------------------------------------------------------------- 3)（S）-3-O-香叶基香叶基甘油磷酸合酶（GGGPS） 是从红毛藻中分离出来的，参与红毛藻细胞壁特有的化合物的合成。 我们想看看这个机制 这种独特类型的酶。 3个数据集-它们已在我们的旋转阳极上冻结和预筛选，并存储用于旅行。 还有其他一些类似的突变体正在结晶。 如果我们能得到晶体，预冷冻和预测试它们，如果有时间，我们会带着它们沿着收集。 ------------------------------------------------------------------ 我们名单上的每一颗水晶都被预先冷冻和筛选过 我们的旋转阳极 我们会带一些室温的盘子，但是 纯粹作为后备 VraR上的MAD数据是我们此行的首要任务