CRYSTALLOGRAPHIC STUDIES OF METALLOPROTEINS (NIKR, BIOB, PFLAE, AND HPPE)
CRYSTALLOGRAPHIC STUDIES OF METALLOPROTEINS (NIKR, BIOB, PFLAE, AND HPPE)
批准号:
7954519
负责人:
PETER GOLDMAN
金额:
$0.02万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2009
资助国家:
美国
项目状态:
已结题
起止时间:
2009-03-01 至 2010-02-28
关键词:
AcidsAnabolismAntibioticsBiotinCell physiologyComputer Retrieval of Information on Scientific Projects DatabaseDNA-Binding ProteinsEnzymesFosfomycinFundingGrantInstitutionIronLaboratoriesMetabolismMetalloproteinsMetalsMononuclearNickelPropertyProtein BindingProteinsPublishingResearchResearch PersonnelResourcesSiteSourceStructureUnited States National Institutes of HealthWorkX-Ray Crystallographybiotin synthaseepoxidaseformate C-acetyltransferaseformate acetyltransferase activating enzymemetalloenzymestructural biologysynchrotron radiationtranscription factoruptake
中文摘要
这个子项目是众多研究子项目之一
英文摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
Metals and metalloproteins are essential for many cellular processes. Our laboratory uses macromolecular X-ray crystallography to study the structure and function of metalloproteins. These proteins include nickel regulatory transcription factor NikR, a DNA-binding protein that regulates cellular nickel uptake; the radical S-adenosylmethionine (SAM) enzymes biotin synthase (BioB), involved in biotin biosynthesis, and pyruvate formate-lyase (PFL) activating enzyme (PflAE), involved in anaerobic metabolism by formation of a protein bound glycyl radical on PFL; and hydroxypropylphosphonic acid epoxidase (HppE), which uses a mononuclear iron site to catalyze a unique epoxidation in the formation of the antibiotic fosfomycin. We have solved and published initial crystal structures of all of these proteins, and some of this work was carried out previously at SSRL. We are now in pursuit of further crystallographic characterization of these metalloenzymes in multiple states to more fully understand their functional properties.
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CRYSTALLOGRAPHIC STUDIES OF METALLOPROTEINS (NIKR, BIOB, PFLAE, AND HPPE)
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批准号:8362216
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项目类别:
-
资助金额:$0.03万
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财政年份:2011
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负责人:PETER GOLDMAN
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依托单位:
CRYSTALLOGRAPHIC STUDIES OF METALLOPROTEINS (NIKR, BIOB, PFLAE, AND HPPE)
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批准号:8170177
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项目类别:
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资助金额:$0.41万
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财政年份:2010
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负责人:PETER GOLDMAN
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依托单位:
Optimizing Therapeutic Ratios for Herbs
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批准号:6334338
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项目类别:
-
资助金额:$23.83万
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财政年份:2000
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负责人:PETER GOLDMAN
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依托单位:
Optimizing Therapeutic Ratios for Herbs
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批准号:6375446
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项目类别:
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资助金额:$18.79万
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财政年份:2000
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负责人:PETER GOLDMAN
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依托单位:
Optimizing Therapeutic Ratios for Herbs
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批准号:6775685
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项目类别:
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资助金额:$12.6万
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财政年份:2000
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负责人:PETER GOLDMAN
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依托单位:
Optimizing Therapeutic Ratios for Herbs
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批准号:6512111
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项目类别:
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资助金额:$12.55万
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财政年份:2000
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负责人:PETER GOLDMAN
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依托单位:
Optimizing Therapeutic Ratios for Herbs
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批准号:6666865
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项目类别:
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资助金额:$12.28万
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财政年份:2000
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负责人:PETER GOLDMAN
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依托单位:
CARCINOGEN METABOLISM BY HOST INTESTINAL BACTERIA
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批准号:3172740
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项目类别:
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资助金额:$16.88万
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财政年份:1982
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负责人:PETER GOLDMAN
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依托单位:
CARCINOGEN METABOLISM BY HOST INTESTINAL BACTERIA
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批准号:3172746
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项目类别:
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资助金额:$18.71万
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财政年份:1982
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负责人:PETER GOLDMAN
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依托单位:
CARCINOGEN METABOLISM BY HOST INTESTINAL BACTERIA
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批准号:3172743
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项目类别:
-
资助金额:$17.72万
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财政年份:1982
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负责人:PETER GOLDMAN
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依托单位:
CARCINOGEN METABOLISM BY HOST INTESTINAL BACTERIA
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批准号:3172744
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项目类别:
-
资助金额:$18.0万
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财政年份:1982
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负责人:PETER GOLDMAN
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依托单位:
CARCINOGEN METABOLISM BY HOST INTESTINAL BACTERIA
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批准号:3172745
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项目类别:
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资助金额:$17.83万
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财政年份:1982
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负责人:PETER GOLDMAN
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依托单位:
PILOT STUDY--CARRAGEENAN DEGRADATION BY FLORA AND COLONIC ULCERS
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批准号:3917805
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:PETER GOLDMAN
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依托单位:
PILOT STUDY--CARRAGEENAN DEGRADATION BY FLORA AND COLONIC ULCERS
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批准号:3940713
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:PETER GOLDMAN
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依托单位:
PILOT STUDY--CARRAGEENAN DEGRADATION BY FLORA AND COLONIC ULCERS
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批准号:4689763
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:PETER GOLDMAN
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依托单位:
PILOT STUDY--CARRAGEENAN DEGRADATION BY FLORA AND COLONIC ULCERS
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批准号:3964547
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:PETER GOLDMAN
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依托单位:
海外基金