MITOCHONDRIAL SWIRLS

线粒体漩涡

• 批准号: 7954589
• 负责人: SEYMOUR BENZER
• 金额: $ 1.12万
• 依托单位: WADSWORTH CENTER
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-02-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954589
• 关键词: Biological; Computer Retrieval of Information on Scientific Projects Database; Crista ampullaris; Data; Drosophila genus; Funding; Grant; Image; Imagery; Institution; Membrane; Methods; Mitochondria; Modeling; Muscle Mitochondria; Oxidative Stress; Peripheral; Publications; Reporting; Research; Research Personnel; Resources; Services; Shapes; Source; Staging; Structure; Surface; Tissues; United States National Institutes of Health; Work; abstracting; animation; high voltage electron microscopy; reconstruction; response; tomography

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. ABSTRACT: We see swirls in Drosophila flight muscle mitochondria in response to oxidative stress. 3D tomography is needed to see their detailed structure. Specifically, we want to know whether there are single or multiple cristae involved in swirl formation, how the swirl shape relates to the shape of normal cristae in these mitochondria, and whether the swirl cristae are attached to the peripheral region of the inner membrane (i.e. whether there are crista junctions). In the 2006-2007 reporting period, tissue blocks were received and sections were cut for HVEM tomography. Two single-tilt reconstructions were made. In the previous reporting period, this project represented the majority of the effort on service projects this year, carried out by Karolyn Buttle, amounting to almost two month's work. Dr. Mannella worked with Ms. Buttle in visualizing the tomographic data collected previously in order to faithfully represent and display the unique structures in these mitochondria. Many different methods were tried, and a variety of illustrations and animations were made for presentation and publication. New images and three new tomographic reconstructions were recorded, in an effort to collect different stages of swirl formation. Considerable effort was again spent on visualization of this data, yielding informative surface-rendered models and animations that were used in some of Dr. Mannella's presentations.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 抽象的： 我们看到果蝇飞行肌肉线粒体的漩涡响应氧化应激。需要3D断层扫描以查看其详细结构。 具体而言，我们想知道漩涡形成中是否涉及单个或多个cristae，漩涡状与这些线粒体中正常cr的形状如何相关，以及是否连接到内膜内膜周围区域（即是否有Crista连接））。 在2006 - 2007年的报告期间，收到组织块，并切割了HVEM断层扫描的切片。 进行了两次单倾斜重建。 在上一个报告期间，该项目代表了卡罗琳·巴特尔（Karolyn Buttle）今年在服务项目上的大部分努力，相当于近两个月的工作。 Mannella博士与Buttle女士合作，可视化先前收集的层析成像数据，以忠实地代表和显示这些线粒体中的独特结构。 尝试了许多不同的方法，并制作了各种插图和动画以进行演示和出版。 记录了新的图像和三个新的断层造影重建，以收集不同阶段的漩涡形成。 再次花费了大量的努力，用于可视化这些数据，从而产生了曼内拉博士的一些演示中使用的内容丰富的表面渲染模型和动画。