SOFTWARE DISTRIBUTION: TCASIM, TCACALC, TCAFLUX

软件分发：TCASIM、TCACALC、TCAFLUX

• 批准号: 7956970
• 负责人: MARK JEFFREY
• 金额: $ 1.78万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7956970
• 关键词: Amino Acids; Biochemical Pathway; Citric Acid Cycle; Companions; Complex; Computer Retrieval of Information on Scientific Projects Database; Computer software; Data; Funding; Gluconeogenesis; Glucose; Glycolysis; Grant; Institution; Ketones; Label; Mass Spectrum Analysis; Metabolic; Metabolism; Pathway interactions; Progress Reports; Relative (related person); Research; Research Personnel; Resources; Series; Source; Time; United States National Institutes of Health; base; in vivo; programs; simulation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Three programs have been developed by the Resource and are used on a daily basis. tcaSIM allows the simulation of complex metabolic networks including the citric acid cycle, glycolysis, gluconeogenesis and anaplerosis. The operator controls all aspects of 13C labeling and fluxes in all pathways. The program generates 13C NMR and HMQC-TOCSY spectra, as well as mass and tandem mass spectra, of numerous metabolites such as amino acids, ketones and glucose. The companion program tcaCALC is used to analyze experimental data, determining the same metabolic parameters as used in tcaSIM from observed NMR or mass spectra. Both these rograms examine the relative flux through pathways, while tcaFLUX determines absolute fluxes from time series of 13C NMR and HMQC-TOCSY spectra. The details of tcaSIM, tcaCALC, and tcaFLUX have been described in previous progress reports.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 资源已经开发了三个程序，并每天使用。 TCASIM允许模拟复杂的代谢网络，包括柠檬酸循环，糖酵解，糖异生和无链球菌。操作员控制所有途径中13C标记和通量的所有方面。该程序产生了13C NMR和HMQC-Tocsy光谱，以及许多代谢产物（例如氨基酸，酮和葡萄糖）的质量和串联质谱。伴随程序TCACALC用于分析实验数据，确定与观察到的NMR或质谱中TCASIM相同的代谢参数。 这两个Rograms都检查了通过途径的相对通量，而TCAFLUX决定了13C NMR和HMQC-Tocsosy光谱的时间序列中的绝对通量。 先前的进度报告中已经描述了TCASIM，TCACALC和TCAFLUX的细节。