FRET DETECTION OF MATING MAPK ACTIVATION

交配图激活的 FRET 检测

• 批准号: 7956539
• 负责人: LEE S BARDWELL
• 金额: $ 0.27万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7956539
• 关键词: Computer Retrieval of Information on Scientific Projects Database; Fluorescence; Fluorescence Resonance Energy Transfer; Funding; Grant; In Vitro; Institution; Laboratories; MAP Kinase Gene; Partner in relationship; Protocols documentation; Reporter; Research; Research Personnel; Resources; Saccharomyces cerevisiae; Signal Transduction; Source; United States National Institutes of Health; Yeasts; base; in vivo; vector

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A FRET based vector was constructed as a reporter for MAPK activation in vitro. The purpose of this project is to develop a protocol for using the FRET reporter in vivo to detect activation of mating MAPK signal cascade in yeast S. cerevisiae.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 构建了基于FRET的MAPK体外激活报告载体。本项目的目的是建立一种在体内使用FRET报告器来检测酿酒酵母交配MAPK信号级联激活的方法。