UNDERSTANDING RECEPTOR AND G PROTEIN INTERACTIONS IN LIVE CELLS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 受体的膜组织可以被各种膜蛋白改变。小窝蛋白1是这类蛋白中最重要的成员之一，它与许多其他蛋白质广泛相互作用，并已知形成膜结构域。我在表达荧光蛋白的活细胞中进行了FCS，scanning-FCS，RICS和N&B分析，以了解小窝蛋白1如何改变受体的膜组织。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Membrane organization of receptors can be altered by various membrane proteins. Caveolin1 is one of the most important members of this class that widely interacts with many other proteins and is known to form membrane domains. I have performed FCS, scanning-FCS, RICS and N&B analysis in live cells expressing fluorescent proteins to understand how caveolin1 alters membrane organization of receptors.