DOMAINS DISTRIBUTION IN CHO CELLS EXPRESSING DELTA-9 DESATURASE

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 我们计划分析增加脂肪酸不饱和度含量对质膜磷脂的分布和共存的影响。我们想研究表达去饱和酶的突变型 CHO 细胞和野生型 CHO 细胞。我们计划使用的技术将是 Laurdan 广义极化。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We planned to analyze the effect of increasing fatty acid unsaturation contents on phospholipids at the plasma membrane, in the domain distribution and coexistence. We would like to study mutant CHO cells expressing a desaturase and the wild type CHO cells. The techniques we are planing to use will be Laurdan generalized Polarization.