DOMAINS DISTRIBUTION IN CHO CELLS EXPRESSING DELTA-9 DESATURASE

表达 Delta-9 去饱和酶的 CHO 细胞中的域分布

• 批准号: 7956563
• 负责人: John M Rimoldi
• 金额: $ 9.47万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7956563
• 关键词: Cell membrane; Chinese Hamster Ovary Cell; Computer Retrieval of Information on Scientific Projects Database; Fatty Acids; Fluorescence; Funding; Grant; Institution; Laboratories; Phospholipids; Research; Research Personnel; Resources; Source; Stearoyl-CoA Desaturase; Techniques; United States National Institutes of Health; desaturase; laurdan; mutant

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We planned to analyze the effect of increasing fatty acid unsaturation contents on phospholipids at the plasma membrane, in the domain distribution and coexistence. We would like to study mutant CHO cells expressing a desaturase and the wild type CHO cells. The techniques we are planing to use will be Laurdan generalized Polarization.

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们计划分析增加脂肪酸不饱和度含量对磷脂在质膜上的分布和共存的影响。我们想研究表达去饱和酶的突变CHO细胞和野生型CHO细胞。我们计划使用的技术将是劳登广义极化。