IMAGING OF PRE-NEOPLASTIC LESIONS IN A MOUSE MODEL OF MEDULLOBLASTOMA

髓母细胞瘤小鼠模型中肿瘤前病变的成像

• 批准号: 7956907
• 负责人: Robert J. Wechsler-Reya
• 金额: $ 0.55万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7956907
• 关键词: A Mouse; Animals; Cerebellum; Characteristics; Computer Retrieval of Information on Scientific Projects Database; Development; Funding; Goals; Grant; Histology; Human; Image; Institution; Lesion; Microscopy; Modeling; Mus; Mutant Strains Mice; Perfusion; Phase; Prevalence; Research; Research Personnel; Resolution; Resources; Shapes; Source; Surface; United States National Institutes of Health; in vivo; insight; interest; medulloblastoma; mouse model; neoplastic; neoplastic cell; research study; tumor; tumor progression

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of our experiments is to image pre-neoplastic lesions in patched mutant mice, a model for human medulloblastoma. Our previous studies indicate that 75-85% of these animals have pre-neoplastic cells on the surface of their cerebellum, although only 15% will go on to develop full-blown tumors. We are interested in determining the size, shape and prevalence of these lesions, and in tracking them to determine what happens to them when they do not become tumors. These studies will involve two phases. Phase I: MR histology to determine the characteristics of pre-neoplastic lesions and the sensitivity and resolution with which they can be detected. This will involve high-resolution imaging of 4-6 mice after perfusion. Phase II. Serial in vivo imaging of 4-6 animals to track the disappearance of pre-neoplastic lesions and/or the development of these lesions into tumors. The feasibility of Phase II will be determined during Phase I. Together these studies will provide important insight into the mechanisms of tumor progression in medulloblastoma.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们实验的目标是对打补丁的突变小鼠的肿瘤前病变进行成像，这是人类髓母细胞瘤的模型。我们之前的研究表明，这些动物中有75%-85%的小脑表面有肿瘤前细胞，尽管只有15%会继续发展为完全发育的肿瘤。我们有兴趣确定这些病变的大小、形状和患病率，并对它们进行跟踪，以确定当它们没有变成肿瘤时会发生什么。这些研究将分两个阶段进行。第一阶段：MR组织学，以确定肿瘤前病变的特征以及它们可以被检测到的敏感度和分辨率。这将涉及到在灌流后对4-6只小鼠进行高分辨率成像。第二阶段：对4-6只动物进行连续活体成像，以跟踪肿瘤前病变的消失和/或这些病变发展为肿瘤的过程。第二阶段的可行性将在第一阶段确定，这些研究将为髓母细胞瘤的肿瘤进展机制提供重要的见解。