NIR ENZYME-ACTIVATED FLUOROGENIC PHOSPHOLIPIDS FOR MULTI-MODALITY IMAGING

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 磷脂酶催化膜磷脂的水解，进而产生一种 多种脂类第二信使。这些脂质衍生产品对这两种疾病都是必要的 生理和病理过程。理解磷脂代谢，因为它 与癌症有关，对于诊断应用和开发新的靶向是必要的 化疗。传统上，脂质代谢物的变化是通过磁学观察的。 共振光谱(MRS)或通过层析方法。使用MRS的组合 利用近红外(NIR)荧光分子探针，我们瞄准了磷脂酶功能 目标是描绘体内特定的细胞信号通路。所获得的信息 分子成像方法可能导致更早的诊断和改善 疾病的管理。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Phospholipases catalyze the hydrolysis of membrane phospholipids, which, in turn, generates a variety of lipid second messengers. These lipid-derived products are necessary for both physiological and pathological processes. An understanding of phospholipid metabolism, as it relates to cancer, is necessary for diagnostic applications and for developing novel targeted chemotherapy. Traditionally, lipid metabolite changes have been observed by magnetic resonance spectroscopy (MRS) or by chromatographic methods. Using a combination of MRS with near-infrared (NIR) fluorescent molecular probes we are targeting phospholipase function with the goal of delineating specific cell signaling pathways in vivo. The information gained from a molecular imaging approach may lead to earlier diagnoses and improved management of disease.