Imaging Core

成像核心

• 批准号: 10333069
• 负责人: EDWARD J DELIKATNY
• 金额: $ 20.23万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-16 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10333069
• 关键词: Animals; Antigens; Back; Benchmarking; Biodistribution; Biopsy; Biopsy Specimen; Bronchoscopy; Calibration; Cancer Patient; Cancerous; Canis familiaris; Carcinoma in Situ; Clinic; Clinical; Clinical Trials; Communities; Contrast Media; Data; Data Analyses; Data Collection; Deposition; Detection; Development; Devices; Diagnostic; Drug Kinetics; Dyes; Equipment; Excision; Faculty; Fibroblasts; Fluorescence; Goals; Histopathology; Human; Image; Image-Guided Surgery; In Situ; Lice; Location; Lung; Lung Neoplasms; Lymph Node Dissections; Maintenance; Malignant Neoplasms; Malignant neoplasm of lung; Measurement; Methods; Micrometastasis; Microscope; Microscopic; Microscopy; Mission; Modeling; Mus; Near-infrared optical imaging; Neoplasm Metastasis; Nodule; Non-Small-Cell Lung Carcinoma; Operative Surgical Procedures; Optical Biopsy; Optics; Outcome; Pathology; Patients; Prognosis; Program Research Project Grants; Protocols documentation; Radiology Specialty; Resolution; Resources; Sampling; Sensitivity and Specificity; Series; Specimen; Surgical Management; Surgical Pathology; Systems Development; Techniques; Testing; Tissue imaging; Tracer; Training; Translating; Translations; Veterinary Schools; animal imaging; cancer clinical trial; cancer imaging; carbonate dehydratase; clinical imaging; computerized data processing; design; digital pathology; ex vivo imaging; fibroblast-activating factor; fluorescence imaging; human imaging; imaging agent; imaging facilities; imaging platform; imaging study; imaging system; improved; instrumentation; lymph node biopsy; lymph nodes; medical schools; microscopic imaging; molecular imaging; molecular pathology; novel; optical imaging; pre-clinical; preclinical imaging; programs; targeted imaging; tissue processing; tumor; tumor microenvironment

Project Summary/Abstract The Imaging Core (Core B) provides centralized imaging support to all levels of this Program Project grant, from small animals to human imaging. The Core combines resources from the Small Animal Imaging Facility in Radiology, the Matthew J Ryan Veterinary School of Medicine and the Center for Precision Surgery to create a facility with state-of-the-art instrumentation, and dedicated faculty and staff to apply NIR optical imaging to intraoperative pulmonary surgery. The Core will focus on developing and implementing back table imaging protocols for fluorescence imaging of tumors in situ and in surgical specimens. The goals are to improve co- registration of fluorescence images with histopathology, accurately determine tumor margin detection and detect micrometastases, secondary tumor deposits and cancerous lymph nodes. We will apply these techniques to pulmonary tumor resections, bronchoscopies, and lymph node specimens. In addition, Core B will provide imaging support to both large animal (canine) and small animal (mouse) components of this program to aid in the translation of the new novel targeted imaging agents into the operating theater in patients with pulmonary cancers. Part of the mission of the Core is to refine tissue processing protocols in order to co-register macro and microscopic tissue images between surgical images and pathology. In doing so we will compare the sensitivity and resolution of different imaging platforms and determine the sensitivity of NIR imaging and the depth of detectable fluorescence. The Core facilitates testing of new imaging cameras, calibrating against fluorescent standards and phantoms, and benchmarking to existing clinical and preclinical imaging scanners. Our goal is to establish a paradigm bridging surgical imaging and pathology for the establishment of an optical biopsy for precise detection of margins in the operating theater. These advances will improve the surgical management and prognosis of lung cancer patients.

项目概要/摘要 成像核心（核心 B）为本计划项目拨款的各个级别提供集中成像支持，从 小动物到人类成像。该核心结合了小动物成像设施的资源 放射学、马修·J·瑞安兽医医学院和精密外科中心创建了一个 配备最先进的仪器和专门的教职员工，将近红外光学成像应用于 术中肺部手术。核心将专注于开发和实施后台成像 原位肿瘤和手术标本荧光成像方案。目标是改善合作 荧光图像与组织病理学配准，准确判断肿瘤边缘检测并检测 微转移、继发性肿瘤沉积和癌性淋巴结。我们将应用这些技术 肺部肿瘤切除术、支气管镜检查和淋巴结标本。此外，Core B 将提供 对该计划的大型动物（犬）和小动物（小鼠）部分提供成像支持，以帮助 将新型靶向显像剂引入手术室治疗肺结核患者 癌症。核心任务的一部分是完善组织处理协议，以便共同注册宏 以及介于手术图像和病理之间的显微组织图像。在此过程中，我们将比较 不同成像平台的灵敏度和分辨率，并确定近红外成像的灵敏度和 可检测荧光的深度。该核心有助于测试新成像相机，并根据 荧光标准和模型，以及现有临床和临床前成像扫描仪的基准测试。 我们的目标是建立一个连接手术成像和病理学的范例，以建立光学 活检以在手术室中精确检测切缘。这些进步将改善手术 肺癌患者的管理和预后。