Imaging Core

项目摘要/摘要 成像核心(核心B)为本计划项目拨款的所有级别提供集中的成像支持，地址为 小动物对人类的想象。核心结合了来自小动物成像设施的资源 放射学，马修·J·瑞安兽医学院和精密外科中心创造了一个 拥有最先进的仪器设备和专职的教职员工，将近红外光学成像应用于 术中肺部手术。核心将专注于开发和实施背部成像 原位和外科标本中肿瘤的荧光成像方案。我们的目标是改善共同 将荧光图像与组织病理学配准，准确确定肿瘤边缘检测和检测 微转移、继发性肿瘤沉积和癌性淋巴结。我们将把这些技术应用于 肺肿瘤切除、支气管镜检查和淋巴结标本。此外，核心B将提供 为该计划中的大动物(犬)和小动物(小鼠)组件提供成像支持，以帮助 新型靶向显像剂在肺癌患者手术中的应用 癌症。核心的部分任务是改进组织处理协议，以便共同注册宏 显微组织图像介于手术图像和病理之间。在此过程中，我们将比较 不同成像平台的灵敏度和分辨率，并决定近红外成像的灵敏度和 可检测到的荧光深度。核心有助于测试新的成像相机，根据 荧光标准和体模，以及现有临床和临床前成像扫描仪的基准。 我们的目标是建立一种连接外科成像和病理的范例，用于建立光学 活检以精确检测手术区的边缘。这些进展将改善外科手术 肺癌患者的治疗和预后。

Project Summary/Abstract The Imaging Core (Core B) provides centralized imaging support to all levels of this Program Project grant, from small animals to human imaging. The Core combines resources from the Small Animal Imaging Facility in Radiology, the Matthew J Ryan Veterinary School of Medicine and the Center for Precision Surgery to create a facility with state-of-the-art instrumentation, and dedicated faculty and staff to apply NIR optical imaging to intraoperative pulmonary surgery. The Core will focus on developing and implementing back table imaging protocols for fluorescence imaging of tumors in situ and in surgical specimens. The goals are to improve co- registration of fluorescence images with histopathology, accurately determine tumor margin detection and detect micrometastases, secondary tumor deposits and cancerous lymph nodes. We will apply these techniques to pulmonary tumor resections, bronchoscopies, and lymph node specimens. In addition, Core B will provide imaging support to both large animal (canine) and small animal (mouse) components of this program to aid in the translation of the new novel targeted imaging agents into the operating theater in patients with pulmonary cancers. Part of the mission of the Core is to refine tissue processing protocols in order to co-register macro and microscopic tissue images between surgical images and pathology. In doing so we will compare the sensitivity and resolution of different imaging platforms and determine the sensitivity of NIR imaging and the depth of detectable fluorescence. The Core facilitates testing of new imaging cameras, calibrating against fluorescent standards and phantoms, and benchmarking to existing clinical and preclinical imaging scanners. Our goal is to establish a paradigm bridging surgical imaging and pathology for the establishment of an optical biopsy for precise detection of margins in the operating theater. These advances will improve the surgical management and prognosis of lung cancer patients.