ROLE OF YPT GTPASES IN INTRACELLULAR TRAFFICKING

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 YPT/Rab GTP酶是细胞内转运的所有步骤中保守的关键调节因子。他们的行动是在GTP“开”和GDP“关”状态之间循环。这种循环依次受到上游监管机构的监管：GEF和GAP。在“开”状态下，YPT/RABS与介导囊泡运输的效应器相互作用。我们聚焦于Ypt31/32，Ypt/Rab GTP酶负责分泌囊泡退出跨高尔基体网络，寻找它们的效应物及其作用方式，以及它们的上游调控因子。我们希望为这些目的使用FRET。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ypt/Rab GTPases are conserved key regulators of all steps of intracellular trafficking. They act by cycling between the GTP "on" and the GDP "off" states. This cycling is regulated in turn by upstream regulators: GEFs and GAPs. At the "on" state Ypt/Rabs interact with effectors that mediate vesicular transport. We focus on Ypt31/32, Ypt/Rab GTPases responsible for secretory vesicle exit from trans-Golgi network, looking for their effectors and their mode of action on these effectors, as well as their upstream regulators. We would like to use FRET for these purposes.