ROLE OF YPT GTPASES IN INTRACELLULAR TRAFFICKING

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 Ypt/Rab GTP酶是细胞内运输的所有步骤的保守的关键调节剂。 它们通过在GTP“开”和GDP“关”状态之间循环来起作用。 这一循环又受到上游调节者的调节：环境基金和全球行动计划。 在“开启”状态下，Ypt/Rabs与介导囊泡转运的效应子相互作用。 我们重点研究了Ypt 31/32，Ypt/Rab GTP酶，寻找它们的效应子及其作用方式，以及它们的上游调控因子。我们希望将FRET用于这些目的。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ypt/Rab GTPases are conserved key regulators of all steps of intracellular trafficking. They act by cycling between the GTP "on" and the GDP "off" states. This cycling is regulated in turn by upstream regulators: GEFs and GAPs. At the "on" state Ypt/Rabs interact with effectors that mediate vesicular transport. We focus on Ypt31/32, Ypt/Rab GTPases responsible for secretory vesicle exit from trans-Golgi network, looking for their effectors and their mode of action on these effectors, as well as their upstream regulators. We would like to use FRET for these purposes.