BUILDING STRUCTURAL MODELS FOR THE RSC CHROMATIN REMODELING COMPLEX

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 在细胞分裂过程中，染色质重塑（染色质的局部和整体结构变化）对于转录和染色体分离都是必要的。在真核生物中，ATP依赖的染色质重塑是由调节DNA-核小体相互作用的大型多蛋白组装体进行的。我们正在努力完善酵母（酿酒酵母）中多蛋白RSC（重塑染色质结构）复合物的结构模型，这是一种ATP依赖的染色质重塑剂，在人类和高等真核生物中具有同源对应物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During cell division, chromatin remodeling (local and global structural changes in chromatin) is necessary for both transcription and chromosomal segregation. In eukaryotes, ATP-dependent chromatin remodeling is performed by large multi-protein assemblies that modulate DNA-nucleosome interactions. We are working to refine structural models of the multi-protein RSC (Remodels the Structure of Chromatin) complex in yeast (Saccharomyces cerevisiae), an ATP-dependent chromatin remodeler with homologous counterparts in humans and higher eukaryotes.