QUESTION OR TRAINING REQUEST FOR THE YEAST RESOURCE CENTER

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我从6000个AD酵母克隆池中获得了一点，发现AD池中的一些细胞能够单独驱动pJD69-4菌株中报告基因ADE和His的激活，而不需要DNA结合结构域的克隆。我想知道这是否正常，我如何解决此问题，以及是否有其他6000个可用克隆池不存在此自动激活问题。感谢您抽出时间回答我的问题。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. I got a bit of the pooled 6000 AD yeast clones and have found that some of the cells within the AD pool are able to drive the activation of the reporter genes ADE and HIS in the pJD69-4 strain alone without the need of the dna binding domain clone. I was wondering if this is normal, how can I address this problem, and is there another pool of the 6000 clones that is available that does not have this autoactivation issue. Thank you for your time in answering my question.