QUESTION OR TRAINING REQUEST FOR THE YEAST RESOURCE CENTER

对酵母资源中心的问题或培训请求

• 批准号: 7957856
• 负责人: Alejandro Chavez
• 金额: $ 0.48万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957856
• 关键词: Address; Binding; Biology; Bite; Cells; Computer Retrieval of Information on Scientific Projects Database; Funding; Fungal Genome; Grant; Institution; Reporter Genes; Research; Research Personnel; Resources; Source; Time; Training; United States National Institutes of Health; Yeasts

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. I got a bit of the pooled 6000 AD yeast clones and have found that some of the cells within the AD pool are able to drive the activation of the reporter genes ADE and HIS in the pJD69-4 strain alone without the need of the dna binding domain clone. I was wondering if this is normal, how can I address this problem, and is there another pool of the 6000 clones that is available that does not have this autoactivation issue. Thank you for your time in answering my question.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我得到了一些汇集的6000个AD酵母克隆，发现AD库中的一些细胞能够单独驱动pJD 69 -4菌株中报告基因ADE和HIS的激活，而不需要DNA结合结构域克隆。 我想知道这是否正常，我如何解决这个问题，以及是否有另一个6000个克隆池没有这个自动激活问题。 谢谢你花时间回答我的问题。