PROTEIN/SIRNA COMPLEX

蛋白质/SIRNA复合物

• 批准号: 7957248
• 负责人: Yu Yuan
• 金额: $ 2.32万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957248
• 关键词: Complex; Computer Retrieval of Information on Scientific Projects Database; Crystallography; Cucumovirus; Double-Stranded RNA; Evolution; Fission Yeast; Funding; Goals; Grant; Host Defense; Human; Immune response; Infection; Influenza A virus; Institution; Light; Molecular; Nature; PTPN11 gene; Proteins; RNA Interference; Research; Research Personnel; Resources; Small Interfering RNA; Small RNA; Source; Structure; Synchrotrons; Tomatoes; United States National Institutes of Health; Viral Proteins; Virus; Virus Diseases; base; influenzavirus; pandemic disease; pathogen; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. RNA silencing is an ancient RNA-based immune response, which is conserved from fission yeast to human beings. In order to anti-virus infection, host uses structural unique small RNAs to pair with the infected virus mRNAs for degradation. However, virus itself has produced diversified RNA silencing suppressors to counter anti-virus response during millions years evolution. The main goal of this proposal is to study this counter-interaction between the host and virus evolved by nature selection at the molecular level. Project 1 Structural Basis for dsRNA Recognition by NS1 protein of Human Influenza Virus A Influenza A viruses are important human pathogens resulting in periodic pandemic threaten, while NS1 protein of influenza A virus (NS1A) shields the virus against host defense. We plan to solve the complex strcuture of Ns1A/siRNA duplex. Project 2 Structural basis for RNA-silencing suppression by Tomato aspermy virus protein 2b 2b protein encoded by cucumovirus functions as post-transcriptional gene silencing (PTGS) suppressor to counter host defense during infection. We plan to solve the complex structure of Tav2b/siRNA duplex.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 RNA沉默是一种古老的基于RNA的免疫反应，从裂变酵母到人类是保守的。为了抗病毒感染，宿主使用结构独特的小RNA与感染的病毒mRNA配对以降解。然而，病毒本身产生了多元化的RNA沉默抑制因子，以抵抗数百万年的进化中的反病毒反应。该提案的主要目的是研究宿主与病毒之间通过分子水平进行自然选择进化的这种反作用。 项目1 dsRNA识别人类流感病毒a的结构基础 流感病毒是重要的人类病原体，导致周期性的大流行威胁，而流感的NS1病毒（NS1A）将病毒避免免受宿主防御的影响。我们计划解决NS1A/siRNA双链体的复杂曲折。 项目2 番茄阿斯波里病毒蛋白2B抑制RNA沉淀抑制的结构基础 2b通过黄瓜病毒编码的蛋白质作为转录后基因沉默（PTGS）抑制剂在感染过程中对抗宿主防御。我们计划解决TAV2B/siRNA双链体的复杂结构。