PROTEIN/SIRNA COMPLEX

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 RNA沉默是一种古老的基于RNA的免疫反应，从裂殖酵母到人类都是保守的。为了抗病毒感染，宿主使用结构独特的小RNA与感染的病毒mRNA配对进行降解。然而，在数百万年的进化过程中，病毒自身产生了多种RNA沉默抑制子来对抗抗病毒反应。本研究的主要目的是在分子水平上研究自然选择进化出的病毒与宿主之间的这种反相互作用。 项目1 A型流感病毒NS 1蛋白识别dsRNA的结构基础 甲型流感病毒（Influenza A virus，简称甲型流感病毒）是人类重要的病原体，可引起周期性的流感大流行，而甲型流感病毒的NS 1蛋白（Influenza A virus，简称NS 1A）可保护病毒免受宿主的防御。我们计划解决Ns 1A/siRNA双链体的复杂结构。 计划2 番茄无精病毒蛋白2b抑制RNA沉默的结构基础 黄瓜花叶病毒编码的2b蛋白是一种转录后基因沉默（PTGS）抑制因子，在感染过程中对抗宿主的防御。我们计划解决Tav 2b/siRNA双链体的复杂结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. RNA silencing is an ancient RNA-based immune response, which is conserved from fission yeast to human beings. In order to anti-virus infection, host uses structural unique small RNAs to pair with the infected virus mRNAs for degradation. However, virus itself has produced diversified RNA silencing suppressors to counter anti-virus response during millions years evolution. The main goal of this proposal is to study this counter-interaction between the host and virus evolved by nature selection at the molecular level. Project 1 Structural Basis for dsRNA Recognition by NS1 protein of Human Influenza Virus A Influenza A viruses are important human pathogens resulting in periodic pandemic threaten, while NS1 protein of influenza A virus (NS1A) shields the virus against host defense. We plan to solve the complex strcuture of Ns1A/siRNA duplex. Project 2 Structural basis for RNA-silencing suppression by Tomato aspermy virus protein 2b 2b protein encoded by cucumovirus functions as post-transcriptional gene silencing (PTGS) suppressor to counter host defense during infection. We plan to solve the complex structure of Tav2b/siRNA duplex.