PROTEIN/SIRNA COMPLEX

蛋白质/SIRNA复合物

• 批准号: 7957248
• 负责人: Yu Yuan
• 金额: $ 2.32万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957248
• 关键词: Complex; Computer Retrieval of Information on Scientific Projects Database; Crystallography; Cucumovirus; Double-Stranded RNA; Evolution; Fission Yeast; Funding; Goals; Grant; Host Defense; Human; Immune response; Infection; Influenza A virus; Institution; Light; Molecular; Nature; PTPN11 gene; Proteins; RNA Interference; Research; Research Personnel; Resources; Small Interfering RNA; Small RNA; Source; Structure; Synchrotrons; Tomatoes; United States National Institutes of Health; Viral Proteins; Virus; Virus Diseases; base; influenzavirus; pandemic disease; pathogen; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. RNA silencing is an ancient RNA-based immune response, which is conserved from fission yeast to human beings. In order to anti-virus infection, host uses structural unique small RNAs to pair with the infected virus mRNAs for degradation. However, virus itself has produced diversified RNA silencing suppressors to counter anti-virus response during millions years evolution. The main goal of this proposal is to study this counter-interaction between the host and virus evolved by nature selection at the molecular level. Project 1 Structural Basis for dsRNA Recognition by NS1 protein of Human Influenza Virus A Influenza A viruses are important human pathogens resulting in periodic pandemic threaten, while NS1 protein of influenza A virus (NS1A) shields the virus against host defense. We plan to solve the complex strcuture of Ns1A/siRNA duplex. Project 2 Structural basis for RNA-silencing suppression by Tomato aspermy virus protein 2b 2b protein encoded by cucumovirus functions as post-transcriptional gene silencing (PTGS) suppressor to counter host defense during infection. We plan to solve the complex structure of Tav2b/siRNA duplex.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 RNA沉默是一种古老的基于RNA的免疫反应，从裂殖酵母到人类都保守。为了抗病毒感染，宿主利用结构独特的小RNA与感染病毒的mRNA配对进行降解。然而，病毒本身在数百万年的进化过程中产生了多样化的RNA沉默抑制子来对抗抗病毒反应。该提案的主要目标是在分子水平上研究宿主和通过自然选择进化而来的病毒之间的这种反相互作用。 项目1 人类甲型流感病毒 NS1 蛋白识别 dsRNA 的结构基础 甲型流感病毒是重要的人类病原体，会导致周期性大流行威胁，而甲型流感病毒（NS1A）的 NS1 蛋白可保护病毒免受宿主防御。我们计划解决 Ns1A/siRNA 双链体的复杂结构。 项目2 番茄不精病毒蛋白 2b 抑制 RNA 沉默的结构基础 黄瓜病毒编码的 2b 蛋白可作为转录后基因沉默 (PTGS) 抑制因子，在感染过程中对抗宿主防御。我们计划解决 Tav2b/siRNA 双链体的复杂结构。