CRYSTAL STRUCTURE OF THE CED-9/CED-4/CED-3 TERNARY COMPLEX

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 细胞凋亡在后生动物的发育和稳态中起着重要的作用。C.细胞程序性死亡（凋亡）的遗传特征。elegans鉴定了四种蛋白质，CED-3、CED-4、CED-9和EGL-1，它们共同控制细胞凋亡的开始。CED-3是一种半胱天冬酶，其激活依赖于CED-4。 CED-9通过未知的机制拮抗CED-4的功能。 CED-3、CED-4和CED-9形成杂寡聚体。阐明C.在elegans中，我们使CED-3/CED-4/CED-9的三元复合物结晶。我们计划通过分子置换或MAD确定结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Apoptosis plays an essential role in the development and homeostasis of metazoans. The genetic characterization of programmed cell death (apoptosis) in C. elegans identified four proteins, CED-3, CED-4, CED-9, and EGL-1, that collectively control the onset of apoptosis. CED-3 is a caspase and its activation depends on CED-4. CED-9 antagonize the function of CED-4 through an unknown mechanism. CED-3, CED-4, and CED-9 form a hetero-oligomer. To elucidate the mechanisms of cell death control in C. elegans, we crystallized the ternary complex of CED-3/CED-4/CED-9. We plan to determine the structure by molecular replacement or by MAD.