X-RAY FIBER DIFFRACTION OF THE OPTIC NERVE HEAD & ITS MICROFIBRILLAR COMPONENTS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 广角X射线散射衍射可以测量组织内纤维的亚微米结构和方向，这可能对功能、疾病发展和计算模型至关重要。在BioCAT对固定的猪眼组织进行WAX，以绘制视神经乳头和周围组织的胶原纤维结构（包括纤维直径、间距、相对丰度和主要方向）。数据分析正在进行中。校准的图像用于计算分子D-间距，丰度和取向的每个原纤维组分，如定性显示。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Wide-angle X-Ray scattering diffraction can measure the submicron structure and orientation of fibers within tissue that may be essential for function, development of disease and computational models. WAX was performed at BioCAT on fixed pig eye tissue to map the collagen fiber structure (including fiber diameter, spacing, relative abundance, and predominate orientation) of the optic nerve head and surrounding tissue. Analysis of the data is underway. The calibrated images are used to calculate the molecular D-spacing, abundance and orientation of each fibril component as qualitatively shown.