ANALYSIS OF MITOCHONDRIAL POOLS OF IRON BY XAS

XAS 分析线粒体铁池

• 批准号: 7954436
• 负责人: Samuel Pazicni
• 金额: $ 0.1万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954436
• 关键词: Computer Retrieval of Information on Scientific Projects Database; Enzymes; Funding; Genetic; Grant; Institution; Iron; Manganese; Mitochondria; Research; Research Personnel; Resources; Source; Superoxide Dismutase; United States National Institutes of Health; Yeasts; human SOD2 protein; mutant; research study; structural biology; synchrotron radiation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Recent genetic experiments have suggested an unprecedented speciation of iron within the mitochrondia of yeast, and in particular have provided evidence for two different pools of iron in mitochondria. This speciation effects the activity of mitochondrial superoxide dismutase (SOD), as iron from one pool is able to displace manganese from the active form of the enzyme, with the other iron pool is SOD-inert. XAS of WT and various mutant yeast mitochondria will be used to provide detailed local structural information for these two pools of mitochrondial iron.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 最近的遗传实验表明，酵母线粒体内存在前所未有的铁形态，特别是为线粒体中两种不同的铁库提供了证据。这种形态影响线粒体超氧化物歧化酶（SOD）的活性，因为来自一个库的铁能够从酶的活性形式中置换锰，而另一个铁库是SOD惰性的。WT和各种突变酵母线粒体的XAS将用于提供这两个线粒体铁池的详细局部结构信息。