ANALYSIS OF MITOCHONDRIAL POOLS OF IRON BY XAS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 最近的遗传学实验表明，酵母的有丝分裂膜中存在前所未有的铁，尤其是为线粒体中存在两种不同的铁池提供了证据。这种形态会影响线粒体超氧化物歧化酶(SOD)的活性，因为一个铁库中的铁能够取代酶的活性形式中的锰，而另一个铁库中的铁是SOD-惰性的。WT的XAS和各种突变的酵母线粒体将被用来为这两个线粒体铁池提供详细的局部结构信息。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Recent genetic experiments have suggested an unprecedented speciation of iron within the mitochrondia of yeast, and in particular have provided evidence for two different pools of iron in mitochondria. This speciation effects the activity of mitochondrial superoxide dismutase (SOD), as iron from one pool is able to displace manganese from the active form of the enzyme, with the other iron pool is SOD-inert. XAS of WT and various mutant yeast mitochondria will be used to provide detailed local structural information for these two pools of mitochrondial iron.