STRUCTURE - FUNCTION AND KINETICS OF AAA+ ATPASES

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 为什么分子机器（AAA ATP酶）的缺陷会导致疾病？这些蛋白质机器将 ATP 水解转化为机械功。人类细胞和致病病原体都利用这项工作来物理操纵蛋白质或 DNA，以拆卸和重新组装膜或其他细胞器、复制 DNA 和遍历细胞分裂、修复受损的蛋白质或调节基因表达。我们不知道这些分子机器如何将 ATP 水解转化为机械功。我们的研究重点是 AAA ATPA 酶的一个子集，即细菌增强子结合蛋白 (EBP)，它们利用 ATP 酶活性来调节有害活动（疾病、作物损害）或有益活动（固氮、环境修复、氢气或其他代谢物生产）所需的基因转录。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Why do defects in molecular machines (AAA+ ATPases) cause disease? These protein machines convert ATP hydrolysis into mechanical work. Both human cells and disease causing pathogens use this work to physically manipulate proteins or DNA to dismantle and reassemble membranes or other organelles, to replicate DNA and traverse cell division, to repair damaged proteins, or to regulate gene expression. We do not know how these molecular machines convert ATP hydrolysis into mechanical work. Our research focuses on one subset of AAA+ ATPAses, the bacterial-enhancer-binding proteins (EBPs) which use their ATPase activities to regulate transcription of genes needed for harmful activites (diseases, crop damage) or helpful ones (nitrogen fixation, environmental remediation, hydrogen or other metabolite production).