STRUCTURE - FUNCTION AND KINETICS OF AAA+ ATPASES

AAA 腺苷酸酶的结构 - 功能和动力学

• 批准号: 7954895
• 负责人: B TRACY NIXON
• 金额: $ 6.52万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954895
• 关键词: ATP Hydrolysis; ATP phosphohydrolase; Biophysics; Cell division; Cells; Computer Retrieval of Information on Scientific Projects Database; DNA; Defect; Disease; Funding; Gene Expression; Genetic Transcription; Grant; Human; Hydrogen; Institution; Kinetics; Mechanics; Membrane; Molecular Machines; Nitrogen Fixation; Organelles; Production; Proteins; Research; Research Personnel; Resources; Source; Structure; United States National Institutes of Health; Work; enhancer binding protein; pathogen; remediation; repaired

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Why do defects in molecular machines (AAA+ ATPases) cause disease? These protein machines convert ATP hydrolysis into mechanical work. Both human cells and disease causing pathogens use this work to physically manipulate proteins or DNA to dismantle and reassemble membranes or other organelles, to replicate DNA and traverse cell division, to repair damaged proteins, or to regulate gene expression. We do not know how these molecular machines convert ATP hydrolysis into mechanical work. Our research focuses on one subset of AAA+ ATPAses, the bacterial-enhancer-binding proteins (EBPs) which use their ATPase activities to regulate transcription of genes needed for harmful activites (diseases, crop damage) or helpful ones (nitrogen fixation, environmental remediation, hydrogen or other metabolite production).

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 为什么分子机器（AAA+ ATPases）的缺陷会导致疾病？这些蛋白质机器将ATP水解转化为机械工作。引起病原体的人类细胞和疾病都使用这项工作来物理操纵蛋白质或DNA来拆除并重新组装膜或其他细胞器，以复制DNA和遍及细胞分裂，以修复受损的蛋白质，或调节基因表达。我们不知道这些分子机器如何将ATP水解转化为机械工作。我们的研究重点是AAA+ ATPases的一个子集，即细菌 - 增强剂结合蛋白（EBP），这些蛋白（EBP）使用其ATPase活动来调节有害活动物所需的基因转录（疾病，作物损害）或有帮助的基因（氮固定，环境修复，氢化，氢化，氢或其他代谢产物）。