NUCLEOTIDE DEPENDENT CONFORMATIONAL CHANGES IN S54-DEPENDENT AAA+ATPASES

S54 依赖的 AAA 腺苷酸酶中核苷酸依赖的构象变化

• 批准号: 7182098
• 负责人: B TRACY NIXON
• 金额: $ 3.25万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7182098
• 关键词: biomedical resource; biophysics; clinical research

DESCRIPTION (provided by applicant): We propose to continue operation of the Biophysics Collaborative Access Team (BioCAT) undulator beamline 18-1D at the Advanced Photon Source, Argonne National Laboratory. It's mission is to provide a national research resource for the study of partially ordered biological molecules, complexes of biomolecules and cellular structures under conditions similar to those present in living cells. The goal of such research is to understand the detailed mechanisms of action of such systems at the molecular level. The techniques employed are non-crystalline x-ray diffraction, x-ray scattering and x-ray absorption emission spectroscopy. The BioCAT facilities provide a resource unique on the continent; only SPring-8 in Japan and the ESRF in France provide similar capabilities. New thrust areas include molecular shape determination from small angle scattering, high-resolution spectroscopy, micro-emission/absorption spectroscopy, and micro-diffraction. Major enhancements are planned for the areas of fiber diffraction, especially for the muscle diffraction program. Research and development efforts are also proposed in several areas directly related to improving data quality, reliability and efficiency of operation. Novel modes of operation are proposed that improve throughput and the quality of training.

描述（由申请人提供）：我们建议继续在阿贡国家实验室先进光子源运行生物物理学协作访问团队 (BioCAT) 波荡器光束线 18-1D。它的使命是为在类似于活细胞中的条件下研究部分有序的生物分子、生物分子复合物和细胞结构提供国家研究资源。此类研究的目标是在分子水平上了解此类系统的详细作用机制。所采用的技术是非晶X射线衍射、X射线散射和X射线吸收发射光谱。 BioCAT 设施提供了非洲大陆独一无二的资源；只有日本的 SPring-8 和法国的 ESRF 提供类似的功能。新的推动领域包括通过小角度散射、高分辨率光谱、微发射/吸收光谱和微衍射确定分子形状。计划对纤维衍射领域进行重大改进，特别是肌肉衍射程序。还建议在与提高数据质量、可靠性和运行效率直接相关的几个领域进行研究和开发工作。提出了新的操作模式来提高吞吐量和训练质量。