TOWARD THE ATOMIC STRUCTURE OF THE NUCLEAR PORE COMPLEX

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 核孔复合体（NPC）是非分裂细胞中穿过核膜到达基因组的唯一通道。因此，研究NPC的原子细节将有助于了解HIV的预整合复合物（PIC）如何与NPC对接并将其基因组传递到细胞核。我们的工作成果将从根本上推动但不限于艾滋病研究。 我们的具体目标是确定核孔复合物（NPC）的原子结构，NPC是进入和离开真核细胞核的唯一通道。NPC是40-60 MDa大小的非常大的大分子组装体。它由大约500个蛋白质分子（核孔蛋白或nups）组成，围绕中心8重旋转轴排列在亚复合物中。由于结晶的组装NPC是不切实际的原因有很多，我们试图阐明第一结构的基本建筑subcomplex和联合收割机结合这一信息，在第二步中获得的结构信息，通过单粒子冷冻电子显微镜。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The nuclear pore complex (NPC) is the only passageway across the nuclear envelope and to the genome in a non-dividing cell. As such, investigating the NPC in atomic detail will help understand how the preinitegration complex (PIC) of HIV docks to the NPC and delivers its genome to the nucleus. Results of our work will advance, but it is not limited to, AIDS research in fundamental ways. Our specific aims are to determine the atomic structure of the nuclear pore complex (NPC), the exclusive gateway into and out of the nucleus of the eukaryotic cell. The NPC is a very large macromolecular assembly of 40-60 MDa size. It is composed of some 500 protein molecules (nucleoporins or nups), arranged in subcomplexes around a central 8-fold rotational axis. Since crystallization of the assembled NPC is impractical for many reasons, we attempt to elucidate first structures of the essential architectural subcomplexes and combine this information in a second step with structural information obtained through single-particle cryo-electron microscopy.