Development of pH-sensitive size reducible nanoparticles for cell nucleus target delivery in multidrug-resistant breast cancer

在2022财年，我计划开发和优化聚合物特性。我用表皮生长因子受体靶向多肽修饰D-α-生育酚聚乙二醇1000琥珀酸酯，并对其偶联情况进行了评价。我成功地制备了TPGS-EGF聚合物，并制备了载药胶束。我们确认了胶束的特性适合下一步的移动。为了将胶束直接输送到核中，我们用150 nm大小的脂质体将胶束封顶。结果，在脂质体中捕获了约10个胶束，载药效率提高了4倍。脂质体含有对pH敏感的部分，释放曲线开始得到证实。

In FY2022, I planned to develop and optimize the polymer characteristic. I modified D-α-Tocopherol polyethylene glycol 1000 succinate (TPGS) with EGF receptor-targeted peptide and evaluated the conjugation. I successfully prepared the TPGS-EGF polymer and formulated drug-loaded micelles. We confirm the micelles' characteristics are suitable to move next step.To deliver the micelles directly into the nucleus, we capped the micelles using a 150 nm size liposome. As a result, about ten micelles are captured in the liposome, and the drug loading efficacy was increased up to 4 times that of the micelles. The liposome included pH-sensitive moieties, and the release profile started to confirm.