Development of pH-sensitive size reducible nanoparticles for cell nucleus target delivery in multidrug-resistant breast cancer

开发用于多重耐药乳腺癌细胞核靶向递送的 pH 敏感尺寸可缩减纳米颗粒

• 批准号: 22K12822
• 负责人: Lee Ruda
• 金额: $ 2.66万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K12822/
• 关键词: size changeable; breast cancer; nanoparticles; Multidrug resistance; pH-sensitive; Nanoparticles

In FY2022, I planned to develop and optimize the polymer characteristic. I modified D-α-Tocopherol polyethylene glycol 1000 succinate (TPGS) with EGF receptor-targeted peptide and evaluated the conjugation. I successfully prepared the TPGS-EGF polymer and formulated drug-loaded micelles. We confirm the micelles' characteristics are suitable to move next step.To deliver the micelles directly into the nucleus, we capped the micelles using a 150 nm size liposome. As a result, about ten micelles are captured in the liposome, and the drug loading efficacy was increased up to 4 times that of the micelles. The liposome included pH-sensitive moieties, and the release profile started to confirm.

于二零二二财政年度，我计划开发及优化聚合物特性。我用EGF受体靶向肽修饰D-α-生育酚聚乙二醇1000琥珀酸酯（TPGS）并评价其偶联性。我成功地制备了TPGS-EGF聚合物并配制了载药胶束。为了将胶束直接递送到细胞核中，我们使用150 nm大小的脂质体将胶束封端。结果，约10个胶束被捕获在脂质体中，并且载药效率增加至胶束的4倍。脂质体包括pH敏感部分，并且释放曲线开始得到证实。

项目成果

Korea Basic Science Institute, Seoul/Kangwon National University/Chonnam National University(韓国)

韩国基础科学研究所，首尔/国立江原大学/国立全南大学（韩国）