FATTY ACID SYNTHASE AND EGFR/HER2 CROSS-TALK IN BREAST CANCER CELLS

乳腺癌细胞中脂肪酸合酶和 EGFR/HER2 的相互作用

• 批准号: 7957019
• 负责人: FRANCISCO J ESTEVA
• 金额: $ 3.25万
• 依托单位: BATTELLE PACIFIC NORTHWEST LABORATORIES
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957019
• 关键词: Affinity Chromatography; Biology; Breast Cancer Cell; Cancer Patient; Cells; Complex; Computer Retrieval of Information on Scientific Projects Database; ERBB2 gene; Enzymes; Epidermal Growth Factor Receptor; Fatty Acids; Fatty-acid synthase; Funding; Grant; Human; Immunoprecipitation; Institution; Ions; Metals; Phosphorylation; Phosphorylation Site; Proteomics; Research; Research Personnel; Resolution; Resources; Signal Transduction; Solid Neoplasm; Source; United States National Institutes of Health; malignant breast neoplasm; outcome forecast; overexpression

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Fatty acid synthase (FASN) is the enzyme that catalyzes the de novo synthesis of fatty acids in cells. FASN is up-regulated in solid tumors, and its overexpression has been associated with poor prognosis in breast cancer patients. FASN regulates the expression of the human epidermal growth factor receptor 2 (her-2 also known as c-erbB-2) gene. We have recently found that FASN forms a complex with phosphorylated EGFR and HER-2. Our hypothesis is that FASN phosphorylation is critical for HER-2 signaling in breast cancer cells. The specific aim of this proposal is to identify the FASN/HER-2 and FASN/EGFR phosphorylation sites in HER2-overexpressing breast cancer cells using immunoprecipitation, immobilized metal-ion affinity chromatography (IMAC), and high resolution LC-MS/MS.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 脂肪酸合成酶（Fatty acid synthase，FATCH）是一种催化细胞内脂肪酸从头合成的酶。 在实体瘤中，FXR上调，并且其过表达与乳腺癌患者的不良预后相关。 人表皮生长因子受体2（her-2，也称为c-erbB-2）基因的表达受FIGURE调节。 我们最近发现，FXR与磷酸化EGFR和HER-2形成复合物。 我们的假设是，在乳腺癌细胞中，FXR磷酸化对HER-2信号传导至关重要。 本提案的具体目的是使用免疫沉淀、固定化金属离子亲和色谱（IMAC）和高分辨率LC-MS/MS鉴定HER 2过表达乳腺癌细胞中的FXR/HER-2和FXR/EGFR磷酸化位点。