FATTY ACID SYNTHASE AND EGFR/HER2 CROSS-TALK IN BREAST CANCER CELLS

乳腺癌细胞中脂肪酸合酶和 EGFR/HER2 的相互作用

• 批准号: 7957019
• 负责人: FRANCISCO J ESTEVA
• 金额: $ 3.25万
• 依托单位: BATTELLE PACIFIC NORTHWEST LABORATORIES
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957019
• 关键词: Affinity Chromatography; Biology; Breast Cancer Cell; Cancer Patient; Cells; Complex; Computer Retrieval of Information on Scientific Projects Database; ERBB2 gene; Enzymes; Epidermal Growth Factor Receptor; Fatty Acids; Fatty-acid synthase; Funding; Grant; Human; Immunoprecipitation; Institution; Ions; Metals; Phosphorylation; Phosphorylation Site; Proteomics; Research; Research Personnel; Resolution; Resources; Signal Transduction; Solid Neoplasm; Source; United States National Institutes of Health; malignant breast neoplasm; outcome forecast; overexpression

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Fatty acid synthase (FASN) is the enzyme that catalyzes the de novo synthesis of fatty acids in cells. FASN is up-regulated in solid tumors, and its overexpression has been associated with poor prognosis in breast cancer patients. FASN regulates the expression of the human epidermal growth factor receptor 2 (her-2 also known as c-erbB-2) gene. We have recently found that FASN forms a complex with phosphorylated EGFR and HER-2. Our hypothesis is that FASN phosphorylation is critical for HER-2 signaling in breast cancer cells. The specific aim of this proposal is to identify the FASN/HER-2 and FASN/EGFR phosphorylation sites in HER2-overexpressing breast cancer cells using immunoprecipitation, immobilized metal-ion affinity chromatography (IMAC), and high resolution LC-MS/MS.

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