MOLECULAR BIOLOGY CORE

分子生物学核心

• 批准号: 7959828
• 负责人: MARK C AUSTIN
• 金额: $ 9.94万
• 依托单位: UNIVERSITY OF MISSISSIPPI MED CTR
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7959828
• 关键词: Biological; Brain; Color; Computer Retrieval of Information on Scientific Projects Database; Computer software; Consultations; Detection; Documentation; Enzymes; Faculty; Funding; Gel; Gene Expression Regulation; Grant; Image Analysis; Imagery; Individual; Institution; Microscope; Molecular; Molecular Biology; Neuraxis; Neuronal Plasticity; Neurons; Neuropeptides; Neurosciences; Neurotransmitters; Process; Research; Research Personnel; Resources; Role; Sampling; Scintillation Counter; Source; Staging; Structure; Synaptic Transmission; System; Systems Analysis; Techniques; Thinking; Time; Training; United States National Institutes of Health; austin; base; cryostat; digital; interest; laser capture microdissection; receptor; relating to nervous system

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Over the past decade, the application of molecular biological techniques to the study of the central nervous system has revolutionized our thinking about synaptic transmission, neuronal plasticity, and neuronal integration, as well as the molecular basis of psychiatric illnesses. The center's Molecular Biology Core emphasizes the application of biotechnologically advanced molecular techniques to study the expression, structure, function, and localization of a variety of neural substrates in the brain. The Molecular Biology Core provides training, consultation, and technical assistance in molecular biological approaches that allow individual researchers to study various molecular endpoints, regulatory mechanisms, as well as the role of transcriptional and translational processes in regulating and controlling the expression of the various neural substrates of interest. Dr. Mark Austin, Core Leader of the Molecular Biology Core, is a molecular neuropharmacologist with expertise in utilizing various molecular biological approaches to study the regulation of gene expression of neurotransmitter enzymes, receptors, transporters and neuropeptides. The Molecular Biology Core provides shared-access to the following amplification, visualization, detection and analysis systems: + Arcturus Veritas Laser Capture Microdissection System + MyiQ Single-Color Real-Time PCR Detection System with SW/Beacon Designer Cycler IQ software + BioRad Gel Documentation System + NanoDrop ND-1000A UV-Vis Spectrophotometer (1-2 ¿l samples) + MCID Elite Image analysis system with Zeiss Axioskop Microscope and Ludl motorized stage + Veritas Microplate Luminometer + Bio-Rad Thermal cycler + BioRad Real-Time PCR Machine + Zeiss Axioskop Digital Photomicroscopy Workstation + GeneQuant RNA/DNA calculator + Packard Scintillation Counter + Leica Cryostat The Molecular Biology Core serves as a central resource that enables, stimulates and facilitates the use of molecular biological approaches and further enhances the methodological diversity of Center Investigators as well as other departmental faculty.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在过去的十年中，分子生物学技术在中枢神经系统研究中的应用彻底改变了我们对突触传递、神经元可塑性和神经元整合以及精神疾病分子基础的认识。该中心的分子生物学核心强调应用生物技术先进的分子技术来研究大脑中各种神经基质的表达，结构，功能和定位。分子生物学核心提供分子生物学方法的培训，咨询和技术援助，使个人研究人员能够研究各种分子终点，调节机制以及转录和翻译过程在调节和控制各种感兴趣的神经底物表达中的作用。 博士Mark Austin是分子生物学核心的核心领导人，是一位分子神经药理学家，擅长利用各种分子生物学方法研究神经递质酶、受体、转运蛋白和神经肽的基因表达调控。 分子生物学核心提供以下扩增、可视化、检测和分析系统的共享访问： + Arcturus Veritas激光显微切割系统 + 配备SW/Beacon Designer Cycler IQ软件的MyiQ单色实时PCR检测系统 + BioRad凝胶文件系统 + NanoDrop ND-1000 A紫外可见分光光度计（1-2升样品） + MCID Elite图像分析系统，配备Zeiss Axioskop显微镜和Ludl电动载物台 + Veritas微孔板光度计 + Bio-Rad热循环仪 + BioRad实时PCR仪 + Zeiss Axioskop数字显微摄影工作站 + GeneQuant RNA/DNA计算器 + 帕卡德闪烁计数器 + Leica低温恒温器 分子生物学核心作为一个中央资源，使，刺激和促进分子生物学方法的使用，并进一步提高中心研究人员以及其他部门的教师的方法多样性。