NEW CHEMICAL TOOLS FOR THE QUANTITATIVE ASSESSMENT OF BIOLOGICAL METABOLOMES

用于生物代谢组定量评估的新化学工具

• 批准号: 7959892
• 负责人: Shane Lamos
• 金额: $ 9.45万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7959892
• 关键词: Biological; Biological Process; Chemicals; Computer Retrieval of Information on Scientific Projects Database; Disease; Equilibrium; Funding; Gene Expression; Genetic; Grant; Institution; Label; Measurement; Metabolic; Play; Reagent; Research; Research Personnel; Resources; Role; Sampling; Source; Spectrum Analysis; Symptoms; System; Systems Biology; Techniques; United States National Institutes of Health; Vermont; biological systems; comparative; enzyme activity; functional group; novel; technology development; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Metabolites play a key role in regulating biological function by activating or inhibiting both gene expression and enzyme activity. Therefore, the quantification of metabolites in a system allows for the comparative analysis of biological samples (systems biology). The development of technologies that allow for the comprehensive quantitative measurement of metabolites will be a crucial first step in restoring metabolic balance and mitigating symptoms of certain diseases. In order to quantify and compare metabolites, they must be selectively labeled with isotopic markers such that molecules are readily recognized by spectroscopy techniques. Novel chemical reagents are herein proposed that will target the most prevalent metabolite functional groups and allow for the quantification of a large number of the key metabolites of biological systems.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 代谢产物通过激活或抑制基因表达和酶活性，在调节生物功能中起着关键作用。因此，系统中代谢物的量化允许对生物样本(系统生物学)进行比较分析。能够对代谢物进行全面定量测量的技术的开发将是恢复代谢平衡和减轻某些疾病症状的关键的第一步。 为了量化和比较代谢物，必须选择性地用同位素标记物标记它们，以便分子容易被光谱学技术识别。本文提出的新型化学试剂将针对最普遍的代谢物官能团，并允许对生物系统的大量关键代谢物进行定量。