PHOTORECEPTORS INVOLVED IN CHLAMYDOMONAS REINHARDTII CIRCADIAN ENTRAINMENT

参与莱茵衣藻昼夜节律牵引的光感受器

• 批准号: 7960118
• 负责人: SIGRID JACOBSHAGEN
• 金额: $ 4.43万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-22 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7960118
• 关键词: Animal Model; Animals; Biochemical; Biological Models; Biomedical Research; Cell physiology; Chlamydomonas reinhardtii; Circadian Rhythms; Computer Retrieval of Information on Scientific Projects Database; Evolution; Funding; Grant; Green Algae; Hand; Human; Insecta; Institution; Mammals; Mouse-ear Cress; Neurospora crassa; Organism; Pacemakers; Photoreceptors; Plants; Research; Research Personnel; Resources; Rhodopsin; Signal Transduction; Source; United States National Institutes of Health; Vascular Plant; circadian pacemaker; cryptochrome; falls; fungus

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The long-term objectives of the proposed research are to elucidate the biochemical mechanisms underlying the circadian clock in Chlamydomonas reinhardtii. This unicellular, eukaryotic green alga serves as a model system for a number of cell processes in animals and plants. Understanding its circadian clock will contribute to an understanding of circadian clock evolution and thus also help to better understand the circadian clock in humans. The strategy of achieving the long-term objectives is to identify the photoreceptor(s) responsible for adjusting the circadian clock to the daily light/dark cycles. If successful, the photoreceptors can be used as a handle to investigate the signal transduction chain(s) that convey the information to the central oscillator and possibly the components of the oscillator itself. C. reinhardtii provides an advantage over other model organisms such as Arabidopsis thaliana because it contains only a limited number of photoreceptors. They fall into three classes: cryptochromes, rhodopsins, and ototropins. Phototropins are blue-light photoreceptors found in higher plants and green algae (Briggs and Christie 2002). Whether phototropins are involved in lightentrainment of the circadian clock is not completely understood yet. Cryptochromes on the other hand are blue-light photoreceptors that were shown to be involved in clock entrainment in higher plants and insects (Cashmore 2003). They are also found in mammals but serve as components of the pacemaker that creates the circadian oscillation in this group of organisms. In fungi as exemplified by Neurospora crassa, cryptochrome seems not to be involved in the circadian clock.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 拟议的研究的长期目标是阐明在Chlamydomonas Reinhardtii中昼夜节律的生化机制。这种单细胞的真核绿藻可作为动物和植物多种细胞过程的模型系统。了解其昼夜节律将有助于理解昼夜节律的演变，从而有助于更好地了解人类的昼夜节律。实现长期目标的策略是确定负责将昼夜节律调整为日常光/黑暗周期的光感受器。如果成功，则可以将光感受器用作调查信号转导链的手柄，这些链将信息传达给中心振荡器以及振荡器本身的组件。 C. reinhardtii比其他模型生物（如拟南芥）提供了优势，因为它仅包含有限数量的感光体。它们分为三类：隐形色素，视紫红蛋白和耳to蛋白。光蛋白是在较高植物和绿色藻类中发现的蓝光光感受器（Briggs and Christie 2002）。尚未完全了解光蛋白是否参与昼夜节律时钟的轻度。另一方面，加密染色体是蓝光光感受器，这些光感受器被证明参与了高等植物和昆虫的时钟夹带（Cashmore 2003）。它们也被发现在哺乳动物中，但作为起搏器的组成部分，在这一生物体中创造了昼夜节律的振荡。在Fungi中，神经孢子虫（Neurospora crassa）的例证，隐形色素似乎没有参与昼夜节律。