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IMMUNE MONITORING LAB

IMMUNE MONITORING LAB
免疫监测实验室
批准号：
7944763
负责人：
LELAND H HARTWELL
金额：
$23.75万
依托单位：
FRED HUTCHINSON CANCER RESEARCH CENTER
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
项目状态：
已结题
起止时间：
2009-03-10 至 2013-12-31
来源：
https://reporter.nih.gov/project-details/7944763
关键词：
Adoptive TransferAffinityAllelesAnalysis of VarianceAntibodiesAntigensB-LymphocytesBindingBiologicalBiological AssayBiological TestingCancer Center Support GrantCancer PatientCell SeparationCell physiologyCell surfaceCellsCellular AssayChromiumCircular DNAClonal ExpansionComplexConsultationsCytolysisCytotoxic T-LymphocytesDNADNA Sequence RearrangementData AnalysesDendritic CellsDevelopmentEnzyme-Linked Immunosorbent AssayEvaluationExcisionExperimental DesignsFlow CytometryFluorescent DyesFluorochromeFrequenciesGel ChromatographyGenetic PolymorphismGenomeGerda brand of difluprednateHLA-A geneHLA-B AntigensHLA-C AntigensHousingHumanHuman ResourcesImmuneImmune responseImmunologic MonitoringImmunologicsImmunologyImmunotherapyIn VitroIndividualKnowledgeLabelLaboratoriesLengthLymphocyteMHC Class I GenesMacacaMalignant NeoplasmsMeasurementMeasuresMediator of activation proteinMethodologyMethodsMicrospheresMitogensMixed Lymphocyte Culture TestMolecularMolecular AnalysisMusNamesNatural Killer CellsNatureOutputPeptide/MHC ComplexPeptidesPerformancePeripheralPeripheral Blood Mononuclear CellPhenotypePopulationProceduresProductionProliferatingProteinsRadioimmunoassayReactionReaderReagentRelative (related person)Request for ApplicationsResearchResearch PersonnelResource SharingResourcesReverse Transcriptase Polymerase Chain ReactionRoleSamplingServicesSpottingsStaining methodStainsStem cell transplantStreptavidinStructureT-Cell ReceptorT-Cell Receptor-Rearrangement Excision DNA CirclesT-LymphocyteTRB@ gene clusterTechniquesTechnologyThymidineThymus GlandTimeTrainingUniversitiesWashingtonantibody conjugatebasebeta Chain Antigen T Cell Receptorbeta-2 Microglobulincell mediated lymphocytolysis testcomplementarity-determining region 3cytokinedesignenzyme linked immunospot assaykillingsmonocytemonomerreconstitutionresponsetool

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中文摘要
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英文摘要
The Immune Monitoring Lab provides investigators with a broad spectrum of services and expertise to carry out a range of assays of human proteins and other biological mediators and perform studies where precise evaluations of the immunologic response are required. Resource services include the production of peptide- MHC Class-l tetramers, unique reagents for accessing antigen-specific T cells; molecular assays for T cell receptor (TCR) related analysis, cellular assays for determining cellular function and analytical services using ELISA and Luminex microbead methodology. This application requests continued support for a resource which fulfills an essential role for research within the Fred Hutchinson/University of Washington Cancer Consortium.
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