Animal Physiology Core

动物生理学核心的使命是协助研究人员在体内评估小鼠和大鼠的葡萄糖和脂肪酸代谢、胰岛素敏感性和能量稳态。它进行身体肥胖分布的研究，并促进核磁共振光谱，功能磁共振成像和microPET分析和成像， 进行代谢研究的实验模型。该中心还为研究者实验室提供专门的啮齿动物手术，以及与糖尿病并发症相关的几项心脏功能评估。 通过与DRTC其他核心的合作，明确了药理学、饮食、环境和遗传改变对葡萄糖稳态、胰岛素作用和代谢的影响。为了实现这些目标，动物生理学核心将：1） 建议研究者设计与啮齿动物中葡萄糖稳态和胰岛素作用控制相关的代谢研究; 2）为研究者提供全身和组织特异性葡萄糖敏感性和胰岛素作用的专门测量，包括但不限于胰岛素、胰腺和高血糖钳夹研究; 3）为胰岛素敏感性研究提供专门的手术模型， 能量平衡，葡萄糖和脂肪酸代谢; 4）为学生，博士后研究员，研究人员和技术人员提供指导，以执行必要的手术和生理技术，以评估葡萄糖稳态和胰岛素作用的控制; 5）提供全身碳水化合物/脂肪酸氧化、能量消耗、进食行为的分析，和自发活动，（间接量热法）和行为啮齿动物笼; 6）提供对自发性的影响的评估，（运行轮）或预定（跑步机）运动对葡萄糖稳态和代谢的影响; 7）向研究者提供使用microCT对啮齿动物脂肪组织分布的专门测量以及使用NMR对肝脏和肌肉中的糖原、肝内脂质和肌细胞内脂质的测量; 8）通过功能性MRI（fMRI）和microPET扫描向研究者提供脑能量和葡萄糖利用的专门测量;和9）进行心脏缺血再灌注损伤和自主神经病变的定量分析。所有这些服务都提供给新的糖尿病研究人员，以及从事糖尿病相关项目的研究人员，这些项目可以通过使用该核心的专业知识和设施来丰富和扩展。

The mission of the Animal Physiology Core is to assist investigators in the in vivo assessment of glucose and fatty acid metabolism, insulin sensitivity and energy homeostasis in mice and rats. It performs studies of body adiposity distribution and facilitates NMR spectroscopy, fMRI and microPET analysis and imaging of experimental models undergoing metabolic studies. The Core also provides specialized rodent surgeries for investigator laboratories and several cardiac functional assessments related to diabetic complications. Through collaborative efforts with the other Cores of the DRTC, the effects of defined pharmacological, dietary, environmental and genetic alterations are thoroughly characterized for their effects on glucose homeostasis, insulin action, and metabolism. To accomplish these goals, the Animal Physiology Core will: 1) advise investigators in designing metabolic studies relevant to the control of glucose homeostasis and insulin action in rodents; 2) make available to investigators specialized measurements of whole body and tissue specific glucose sensitivity and insulin action including, but not limited to insulin, pancreatic and hyperglycemic clamp studies; 3) provide specialized surgical models for the study of insulin sensitivity, energy balance, and glucose and fatty acid metabolism; 4) offer instruction to students, postdoctoral fellows, investigators and technical staff in performing surgical and physiological techniques necessary to evaluate the controls of glucose homeostasis and insulin action; 5) provide analysis of whole body carbohydrate/fatty acid oxidation, energy expenditure, feeding behavior, and locomotor activity using specialized metabolic (indirect calorimetry) and behavioral rodent cages; 6) provide assessment of the effects of spontaneous (running wheel) or scheduled (treadmill) exercise on glucose homeostasis and metabolism; 7) make available to investigators specialized measurements of rodent adipose tissue distribution using microCT and measurements of glycogen in liver and muscle, intrahepatic lipids and intramyocellular lipids using NMR; 8) make available to investigators specialized measurements of brain energy and glucose utilization by functional MRI (fMRI) and microPET scanning; and 9) perform quantitative analyses of cardiac ischemia reperfusion injury and autonomic neuropathy. All these sen/ices are available to investigators new to diabetes research, as well as to investigators working on diabetes-related projects that can be enriched and extended by the use of the expertise and facilities of this core.