Hormone Assay Core

荷尔蒙分析核心的使命是提供各种荷尔蒙的成本效益和效率分析、适当的方法、细胞系和数据存储场所，以促进爱因斯坦DRTC的糖尿病研究工作。在过去的两年里，核心平均每年进行30,000次化验，以支持34名DRTC调查人员。核心的生产力一直很突出，发表了76篇论文 这涉及过去两年半的核心使用情况。此外，在将各种啮齿动物β细胞系提供给爱因斯坦的研究人员和国内外其他研究实验室之前，对其适当的生理分泌功能和胰岛素含量进行了表征。因此，这一核心已成为支持糖尿病相关研究的地方和国家资源。核心利用 核心总监和人员建立的基于RIA、ELISA和Luminex的方法。数据被简单地存档，检测结果通过一个安全的网站提供，调查人员可以访问。这些数据是加密的，对服务器的访问是密码保护的。按存储容量使用计费系统用于收回DRTC预算未涵盖的成本。对调查人员进行适当分析方法的教育是主任的职责，实验室人员指导研究员和 在适当的情况下，在方法学中进行博士后培训。为了实现这些目标，荷尔蒙分析核心将： 1)使用适当的方法，向DRTC研究人员提供各种激素、脂肪因子和细胞因子的准确、经济有效的分析；2)评估爱因斯坦DRTC维持的一系列小鼠β细胞系的胰岛素分泌，供本地使用和分发给科学界；3)提供有效的数据存储和访问系统，向研究人员提供结果并监测核心使用情况； 4)向DRTC研究人员提供有关核心的使用以及在分析性能和数据解释方面的教育方面的专家建议；以及5)将核心作为适当的年轻糖尿病研究人员的培训平台。 未来的目标是通过共享购买一个机器人和兼容条形码的伽马计数器来增强检测自动化，这将用于增强程序的半自动化。所有这些信息都可供糖尿病研究新手以及从事糖尿病相关项目的研究人员使用，这些项目可以通过使用这一核心的专业知识和设施得到丰富和扩展。

The mission of the Hormone Assay Core are to provide cost effective and efficient analyses of a variety of hormones, appropriate methodologies, cell lines and data storage venues to promote the Diabetes research efforts of the Einstein DRTC. Over the past two years, the Core has averaged 30,000 assays annually in support of 34 DRTC investigators. Productivity of the Core has been outstanding with 76 papers published that involved Core usage in the past 2.5 years. In addition, the appropriate physiological secretory function and insulin content of a variety of rodent mouse beta cell lines are characterized prior to supplying them to investigators at Einstein and to other investigator laboratories nationally and internationally. Thus, this Core has functioned as a local and national resource in support of diabetes-related research. The Core utilizies RIA, ELISA, and Luminex-based methodologies that have been established by the Core director and personnel. Data are pemianently archived and the results of assays are made available via a secure website accessible by investigators. These data are encrypted and access to the server is password protected. A charge back system is used to recover costs not covered by the DRTC budget. Education of investigators on the appropriate assay method is the function of the Director, and laboratory personnel instruct fellows and post-docs in the methodologies when appropriate. To accomplish these goals, the Hormone Assay Core will: 1) provide accurate, cost effective assays of a variety of hormones, adipokines and cytokines to DRTC investigators, using appropriate methodologies; 2) assess insulin secretion from a series of mouse beta cell lines maintained by the Einstein DRTC for local use and distribution to the scientific community; 3) provide an effective data storage and access system for providing results to investigators and monitoring Core use; 4) provide expert advice to DRTC investigators on Core use and education in assay performance and data interpretation; and 5) utilize the Core as a training platform for appropriate young diabetes investigators. Future goals are to enhance assay automation through shared purchase of a robot and bar-code compatible gamma counter, which will be used to enhance semi-automation of procedures. All these sen/ices are available to investigators new to diabetes research, as well as to investigators working on diabetes-related projects that can be enriched and extended by the use of the expertise and facilities of this core.