Overhauser Enhanced Magnetic Resonance Imaging (OMRI)

奥豪瑟增强磁共振成像 (OMRI)

• 批准号: 7965338
• 负责人: murali cherukuri
• 金额: $ 37.9万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7965338
• 关键词: Anatomy; Biochemical; Blood Vessels; Cell Nucleus; Chemicals; Contrast Media; Goals; Hypoxia; Image; Label; Magnetic Resonance Imaging; Magnetism; Maps; Metabolic; Monitor; Nature; Oxygen; Perfusion; Pericytes; Protons; Pyruvate; Pyruvates; Pyruvic Acid; Resolution; Signal Transduction; Tissues; Vascular Permeabilities; Water; in vivo; irradiation; molecular imaging; research study; tumor

a) Profile tumors in terms of tumor vascular permeability and oxygenation using in vivo hyperpolarization of water at low fields. Hyperpolarization is usually carried out with the paramagnetic molecule TAM. From the image intensity profiles we obtained tumor oxygen and tumor vascular permeability simultaneously and correlated with immunohistochemical characterization of tumors. We found that tumor hypoxia was associated with increased tumor vascular permeability which in turn was negatively correlated with vascular pericyte coverage. b) Monitor the in vivo breakdown products of hyperpolarized (ex vivo) 13C-labeled pyruvic acid in tumors: In this study, we injected 13C labeled pyruvate and monitored the conversion to lactate. The pyruvate/lactate ratio was quantified in the tumor and correlated with oxygen maps from the tumor obtained from EPRI.

a)利用体内水在低场下的超极化，从肿瘤血管通透性和氧合性方面描绘肿瘤。超极化通常是用顺磁性分子TAM进行的。从图像强度谱中，我们同时获得了肿瘤氧和肿瘤血管通透性，并与肿瘤的免疫组织化学特征相关联。我们发现肿瘤缺氧与肿瘤血管通透性增加有关，而通透性增加又与血管周细胞覆盖率呈负相关。b)监测肿瘤中超极化（离体）13C标记的丙酮酸的体内分解产物：本研究中，我们注射13C标记的丙酮酸并监测其向乳酸的转化。在肿瘤中量化丙酮酸/乳酸比值，并与EPRI获得的肿瘤氧图相关。