ID of Biomarkers in Exhaled Breath Condensates from Asthmatic Patients

哮喘患者呼出气体冷凝物中生物标志物的识别

• 批准号: 7969044
• 负责人: Stewart Levine
• 金额: $ 41.9万
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7969044
• 关键词: Age; Asthma; Biological Markers; Collection; Detection; Devices; Disease; Engineering; Enrollment; Exhalation; Female; Goals; Individual; Laboratories; Mass Spectrum Analysis; Methods; Morbidity - disease rate; National Heart, Lung, and Blood Institute; Patients; Physics; Protocols documentation; Public Health; Refractory; Research Ethics Committees; Risk; Sampling; Specimen; Tube; Universities; airway inflammation; asthmatic patient; disorder control; male; mortality; novel; prevent; volunteer

The aim of this exploratory specimen procurement protocol is to develop a non-invasive method for the detection of asthma-specific biomarkers in exhaled breath condensates (EBC) utilizing a novel collection device that was engineered at the Johns Hopkins University Applied Physics Laboratory. The goal of the study is to identify biomarkers in samples from 3 groups; (i) non-asthmatic normal volunteers, (ii) asthmatic patients whose disease is classified as intermittent or mild, persistent, and (iii) asthmatic patients whose disease is classified as moderate or severe persistent. Each group will consist of 24 patients. These individuals must be between the ages of 18 and 75 and can be male or female. Samples of exhaled breath condensate will be collected using the JHU APL EBC collection device, as well as with the R-Tube. Biomarkers in EBC will be identified by mass spectroscopy. We propose that the identification of biomarkers in EBC that correlate with airway inflammation and disease activity may be used to guide therapy and prevent exacerbations in asthmatic patients. This study was approved by the NHLBI IRB on 2/5/08 and no subjects have been enrolled to date.

本探索性标本采集方案的目的是开发一种非侵入性方法，用于检测呼出气冷凝物（EBC）中的哮喘特异性生物标志物，该方法使用了约翰霍普金斯大学应用物理实验室设计的新型采集装置。 该研究的目的是鉴定来自3组的样品中的生物标志物：（i）非哮喘正常志愿者，（ii）疾病被分类为间歇性或轻度、持续性的哮喘患者，和（iii）疾病被分类为中度或重度持续性的哮喘患者。 每组由24名患者组成。 这些人必须在18至75岁之间，可以是男性或女性。 将使用JHU APL EBC采集装置以及R型管采集呼出气冷凝液样本。 EBC中的生物标志物将通过质谱法鉴定。 我们建议，EBC中与气道炎症和疾病活动相关的生物标志物的鉴定可用于指导治疗和预防哮喘患者的急性发作。 本研究于2008年2月5日获得NHLBI IRB的批准，迄今为止尚未入组受试者。