ID of Biomarkers in Exhaled Breath Condensates from Asthmatic Patients

这一探索性标本采购方案的目的是利用约翰·霍普金斯大学应用物理实验室设计的新型收集设备，开发一种非侵入性方法来检测呼气冷凝液(EBC)中哮喘特异性生物标记物。这项研究的目的是在三组样本中识别生物标志物：(I)非哮喘正常志愿者，(Ii)疾病被归类为间歇性或轻度、持续性的哮喘患者，以及(Iii)哮喘患者，其疾病被归类为中度或重度持续性。每组由24名患者组成。这些人的年龄必须在18岁到75岁之间，可以是男性也可以是女性。呼出的呼吸冷凝液样本将使用JHU APL EBC采集设备以及R-Tube进行采集。EBC中的生物标志物将通过质谱学进行鉴定。我们认为，EBC中与呼吸道炎症和疾病活动性相关的生物标志物的识别可以用于指导治疗和预防哮喘患者的病情恶化。 这项研究于2008年2月5日由NHLBI IRB批准，到目前为止还没有受试者入选。

The aim of this exploratory specimen procurement protocol is to develop a non-invasive method for the detection of asthma-specific biomarkers in exhaled breath condensates (EBC) utilizing a novel collection device that was engineered at the Johns Hopkins University Applied Physics Laboratory. The goal of the study is to identify biomarkers in samples from 3 groups; (i) non-asthmatic normal volunteers, (ii) asthmatic patients whose disease is classified as intermittent or mild, persistent, and (iii) asthmatic patients whose disease is classified as moderate or severe persistent. Each group will consist of 24 patients. These individuals must be between the ages of 18 and 75 and can be male or female. Samples of exhaled breath condensate will be collected using the JHU APL EBC collection device, as well as with the R-Tube. Biomarkers in EBC will be identified by mass spectroscopy. We propose that the identification of biomarkers in EBC that correlate with airway inflammation and disease activity may be used to guide therapy and prevent exacerbations in asthmatic patients. This study was approved by the NHLBI IRB on 2/5/08 and no subjects have been enrolled to date.