ID of Biomarkers in Exhaled Breath Condensates from Asthmatic Patients

本探索性标本采集方案的目的是开发一种非侵入性方法，用于检测呼出冷凝物（EBC）中的哮喘特异性生物标志物，该方法利用约翰霍普金斯大学应用物理实验室设计的新型收集装置。该研究的目的是在三组样本中识别生物标志物；(i)非哮喘的正常志愿者，（ii）疾病分类为间歇性或轻度持续性的哮喘患者，以及（iii）疾病分类为中度或重度持续性的哮喘患者。每组由24名患者组成。这些人必须年龄在18到75岁之间，可以是男性也可以是女性。呼出冷凝水的样本将使用JHU APL EBC收集装置以及r管收集。EBC中的生物标志物将通过质谱法进行鉴定。我们建议在EBC中识别与气道炎症和疾病活动相关的生物标志物可用于指导治疗和预防哮喘患者的恶化。

The aim of this exploratory specimen procurement protocol is to develop a non-invasive method for the detection of asthma-specific biomarkers in exhaled breath condensates (EBC) utilizing a novel collection device that was engineered at the Johns Hopkins University Applied Physics Laboratory. The goal of the study is to identify biomarkers in samples from 3 groups; (i) non-asthmatic normal volunteers, (ii) asthmatic patients whose disease is classified as intermittent or mild, persistent, and (iii) asthmatic patients whose disease is classified as moderate or severe persistent. Each group will consist of 24 patients. These individuals must be between the ages of 18 and 75 and can be male or female. Samples of exhaled breath condensate will be collected using the JHU APL EBC collection device, as well as with the R-Tube. Biomarkers in EBC will be identified by mass spectroscopy. We propose that the identification of biomarkers in EBC that correlate with airway inflammation and disease activity may be used to guide therapy and prevent exacerbations in asthmatic patients. This study was approved by the NHLBI IRB on 2/5/08 and no subjects have been enrolled to date.