Illumina iScan system with processing equipment for the Infinium and GoldenGate B

描述(申请人提供)：本申请的目的是资助购买一个先进和全面的平台，以显著增强H Lee Moffitt癌症中心和研究所的基因组分析能力。Illumina公司的iScan系统和相关的处理设备支持Infinium和GoldenGate磁珠阵列技术。围绕Illumina珠状阵列微阵列系统设计的特定技术使研究人员能够调查新鲜样本、冷冻样本或福尔马林固定石蜡包埋样本的基因组或转录本。DASL技术还允许研究人员使用皮肤和其他表面上皮细胞进行微阵列实验，而不是依赖更具侵入性的技术来获取样本。这种微阵列系统将允许研究人员对基因表达或基因组评估进行高通量、中通量和低通量研究。该系统的灵活性将允许研究人员对每个样本进行高度集中的分析次数，或者增加每个样本的分析次数，直到对基因组或转录组进行全基因组评估。获得这一仪器将使癌症中心的研究人员能够更全面地评估导致个人患癌症的遗传因素，了解单个肿瘤的生理和发育历史，并评估有助于成功治疗癌症的个人和疾病特定因素。该仪器支持的拟议研究项目使用重点调查和全基因组评估来调查可能影响卵巢癌、前列腺癌和肺癌的发病率或严重性的遗传因素。此外，他们还研究了可能导致肺癌发展或乳腺癌复发的表观遗传和转录因子。这些项目刚刚开始描述BeadArray技术可能进行的实验类型。该仪器和支持的技术将极大地扩展莫菲特癌症中心研究人员可用的研究途径，并使人们能够深入描述癌症的基因和基因组变化，这在以前是不可能的。

DESCRIPTION (provided by applicant): The purpose of this application is to fund the purchase of an advanced and comprehensive platform to significantly enhance the genomic analysis capabilities of the H Lee Moffitt Cancer Center and Research Institute. The iScan system and the associated processing equipment from the Illumina Corporation support the Infinium and GoldenGate BeadArray technologies. The specific technologies designed around the Illumina BeadArray microarray system allow investigators to investigate genomes or transcriptomes from fresh samples, frozen samples, or formalin fixed paraffin embedded samples. The DASL technology also allows investigators to use skin and other surface epithelia to perform microarray experimentation rather than rely on more invasive techniques to acquire samples. This microarray system will allow researchers to perform high, medium, and low throughput studies of gene expression or genomic evaluation. The flexibility of the system will allow researchers to perform a highly focused number of assays per sample or increase the number of assays per sample up to the genome-wide evaluation of the genome or transcriptome. The acquisition of this instrument will allow investigators at the Cancer Center to make a more comprehensive evaluation of the genetic factors that predispose individuals to develop cancer, to understand the physiology and developmental history of individual tumors, and to assess the personal and disease specific factors that contribute to the successful treatment of cancer. The proposed research projects supported by this instrument use both focused investigations and genome wide assessments to investigate the genetic factors that might influence the incidence or severity of ovarian cancer, prostate cancer, and lung cancer. Additionally, they look at epigenetic and transcriptional factors that might contribute to the development of lung cancer or the recurrence of breast cancer. These projects just begin to describe the types of experimentation possible with the BeadArray technology. The instrument, and the supported technology, will greatly expand the avenues of investigation available to researchers at the Moffitt Cancer Center and enable depth of characterization of genetic and genomic changes in cancer that has previously not been possible.