Multiphoton Facility for Intravital Imaging

用于活体成像的多光子设施

• 批准号: 7794462
• 负责人: JONATHAN JAMES ART
• 金额: $ 50万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-20 至 2013-07-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794462
• 关键词: AIDS prevention; Animals; Applied Research; Autoimmune Diseases; Axonal Transport; Binding; Biological Models; Cancer Immunology Science; Cell physiology; Cells; Chicago; Collaborations; Complex; Computer software; Custom; Cytoskeletal Proteins; Development; Diabetes Mellitus; Embryo; Endocrine Glands; Event; Fostering; Funding; Housing; Illinois; Image; Image Analysis; Immunology; Institution; Interdisciplinary Study; Laser Microscopy; Life; Malignant Neoplasms; Measurement; Methodology; Nerve Degeneration; Nervous system structure; Neurosciences; Pathogenesis; Pathologic Processes; Physiological; Proteins; Research; Research Personnel; Resources; Scientist; Signal Transduction; Slice; Structure; System; Technology; Tissues; United States National Institutes of Health; Universities; Viral; Virus; base; cell type; insight; instrument; instrumentation; interest; molecular dynamics; molecular imaging; new therapeutic target; optical imaging; programs; research study; trafficking; tumor; virology

DESCRIPTION (provided by applicant): A multiphoton laser microscopy (MPLM) system from Prairie Technologies is requested. The custom system will be assembled in collaboration with the Northwestern University Multiphoton Center Core. The system, developed by Prairie and Northwestern Scientists with software and hardware for simultaneous physiological measurements, consists of two stations: one capable of imaging tissue of live animals, the other for tissue slices. The instrument will be housed in the Research Resources Center (RRC) of the University of Illinois at Chicago. The rapidly expanding Imaging Facility of the RRC provides all research groups at UIC and surrounding institutions access to a full range of EM, optical and image analysis capabilities. The requested MPLM and associated technologies are uniquely suited to imaging of molecules and structures in cells located deep within live tissue sections or living animals. Experiments that require these methodologies cannot be undertaken with the instruments currently at UIC. The intellectual and technological resources necessary to understand complex pathological processes that involve intra and intercellular transport of molecules, development in various model systems, cytoskeletal remodeling, axonal transport, immunology, viral entry, tumor formation, signaling and molecular imaging are in place. The MPLM technology will be critical in advancing our research programs as well as fostering interactions among investigators with diverse but compatible interests. Our overall objective as a group is to understand the dynamic molecular events that occur within the context of specific tissues. This is particularly critical in complex tissues like the nervous system, developing embryos, endocrine organs, where relationships between different cell types cannot be replicated in isolated cultures. MPLM imaging will provide us with heretofore unavailable windows on basic molecular interactions among key proteins involved in intracellular trafficking, cell signaling, and pathogenesis. Our strengths, as an interdisciplinary research group with basic and applied research programs in Development, Cell Physiology, Neuroscience, Virology and Immunology, provide the basis of the need for this instrumentation. The addition of the proposed MPLM system will provide a unique capability relevant to several key NIH funded investigators where we have a nationally-recognized track-record in axonal transport and neurodegeneration, virus entry and trafficking, cytoskeletal protein remodeling, cancer, immunology, signal transduction, and molecular imaging. We believe that the insights gained will have the potential for pioneering new therapeutic targets and strategies in HIV prevention, cancer, autoimmune disease, diabetes, and neurodegeneration.

描述（由申请人提供）：请求来自草原技术的多光子激光显微镜（MPLM）系统。自定义系统将与西北大学多人中心核心合作组装。该系统由草原和西北科学家开发，具有用于同时生理测量的软件和硬件，由两个站组成：一个站点：一个能够对活动物的组织进行成像，另一个用于组织切片。该工具将安置在芝加哥伊利诺伊大学的研究资源中心（RRC）中。 RRC的快速扩展的成像设施为UIC及其周围机构的所有研究小组提供了所有范围的EM，光学和图像分析功能。所需的MPLM和相关技术独特地适合于现场组织切片或活体动物深处的细胞中的分子和结构的成像。目前在UIC的工具无法进行需要这些方法的实验。理解涉及分子内和细胞间运输的复杂病理过程所必需的智力和技术资源，各种模型系统中的发展，细胞骨架重塑，轴突运输，免疫学，病毒输入，肿瘤形成，信号传导和分子成像。 MPLM技术对于推进我们的研究计划以及促进具有多样但兼容兴趣的研究人员的互动至关重要。我们作为一个整体目标是了解在特定组织的背景下发生的动态分子事件。这在神经系统，发展胚胎，内分泌器官等复杂组织中尤其重要，在孤立的培养物中不能复制不同的细胞类型之间的关系。 MPLM成像将为我们提供有关与细胞内运输，细胞信号传导和发病机理有关的关键蛋白基本分子相互作用的迄今无法获得的窗口。作为一个跨学科研究小组，我们的优势在开发，细胞生理，神经科学，病毒学和免疫学方面具有基本和应用研究计划，为需要这种仪器的基础提供了基础。提出的MPLM系统的添加将提供与几个关键的NIH资助研究者相关的独特能力，在该研究人员中，我们在轴突运输和神经退行性，病毒进入和运输，细胞骨架蛋白质重塑，癌症，免疫学，信号转导和分子想象中具有全国认可的轨道记录。我们认为，获得的见解将有可能开创艾滋病毒，癌症，自身免疫性疾病，糖尿病和神经变性的新的治疗靶标和策略。