Multiphoton Facility for Intravital Imaging

用于活体成像的多光子设施

• 批准号: 7794462
• 负责人: JONATHAN JAMES ART
• 金额: $ 50万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-20 至 2013-07-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794462
• 关键词: AIDS prevention; Animals; Applied Research; Autoimmune Diseases; Axonal Transport; Binding; Biological Models; Cancer Immunology Science; Cell physiology; Cells; Chicago; Collaborations; Complex; Computer software; Custom; Cytoskeletal Proteins; Development; Diabetes Mellitus; Embryo; Endocrine Glands; Event; Fostering; Funding; Housing; Illinois; Image; Image Analysis; Immunology; Institution; Interdisciplinary Study; Laser Microscopy; Life; Malignant Neoplasms; Measurement; Methodology; Nerve Degeneration; Nervous system structure; Neurosciences; Pathogenesis; Pathologic Processes; Physiological; Proteins; Research; Research Personnel; Resources; Scientist; Signal Transduction; Slice; Structure; System; Technology; Tissues; United States National Institutes of Health; Universities; Viral; Virus; base; cell type; insight; instrument; instrumentation; interest; molecular dynamics; molecular imaging; new therapeutic target; optical imaging; programs; research study; trafficking; tumor; virology

DESCRIPTION (provided by applicant): A multiphoton laser microscopy (MPLM) system from Prairie Technologies is requested. The custom system will be assembled in collaboration with the Northwestern University Multiphoton Center Core. The system, developed by Prairie and Northwestern Scientists with software and hardware for simultaneous physiological measurements, consists of two stations: one capable of imaging tissue of live animals, the other for tissue slices. The instrument will be housed in the Research Resources Center (RRC) of the University of Illinois at Chicago. The rapidly expanding Imaging Facility of the RRC provides all research groups at UIC and surrounding institutions access to a full range of EM, optical and image analysis capabilities. The requested MPLM and associated technologies are uniquely suited to imaging of molecules and structures in cells located deep within live tissue sections or living animals. Experiments that require these methodologies cannot be undertaken with the instruments currently at UIC. The intellectual and technological resources necessary to understand complex pathological processes that involve intra and intercellular transport of molecules, development in various model systems, cytoskeletal remodeling, axonal transport, immunology, viral entry, tumor formation, signaling and molecular imaging are in place. The MPLM technology will be critical in advancing our research programs as well as fostering interactions among investigators with diverse but compatible interests. Our overall objective as a group is to understand the dynamic molecular events that occur within the context of specific tissues. This is particularly critical in complex tissues like the nervous system, developing embryos, endocrine organs, where relationships between different cell types cannot be replicated in isolated cultures. MPLM imaging will provide us with heretofore unavailable windows on basic molecular interactions among key proteins involved in intracellular trafficking, cell signaling, and pathogenesis. Our strengths, as an interdisciplinary research group with basic and applied research programs in Development, Cell Physiology, Neuroscience, Virology and Immunology, provide the basis of the need for this instrumentation. The addition of the proposed MPLM system will provide a unique capability relevant to several key NIH funded investigators where we have a nationally-recognized track-record in axonal transport and neurodegeneration, virus entry and trafficking, cytoskeletal protein remodeling, cancer, immunology, signal transduction, and molecular imaging. We believe that the insights gained will have the potential for pioneering new therapeutic targets and strategies in HIV prevention, cancer, autoimmune disease, diabetes, and neurodegeneration.

描述（由申请人提供）：需要Prairie Technologies的多光子激光显微镜（MPLM）系统。定制系统将与西北大学多光子中心核心合作组装。该系统由草原和西北科学家开发，具有用于同步生理测量的软件和硬件，由两个站组成：一个能够对活体动物的组织进行成像，另一个用于组织切片。该仪器将被安置在芝加哥伊利诺伊大学的研究资源中心（RRC）。RRC的成像设施迅速扩大，为UIC和周边机构的所有研究小组提供了全方位的EM，光学和图像分析能力。所要求的MPLM和相关技术特别适合于位于活组织切片或活动物深处的细胞中的分子和结构的成像。需要这些方法的实验不能用UIC目前的仪器进行。了解复杂的病理过程所需的知识和技术资源，包括分子的细胞内和细胞间运输，各种模型系统的发展，细胞骨架重塑，轴突运输，免疫学，病毒进入，肿瘤形成，信号传导和分子成像。MPLM技术对于推进我们的研究计划以及促进具有不同但兼容兴趣的研究人员之间的互动至关重要。作为一个团队，我们的总体目标是了解特定组织背景下发生的动态分子事件。这在神经系统、发育中的胚胎、内分泌器官等复杂组织中尤其重要，因为在这些组织中，不同细胞类型之间的关系无法在隔离培养物中复制。MPLM成像将为我们提供迄今为止不可用的窗口，涉及细胞内运输，细胞信号传导和发病机制的关键蛋白质之间的基本分子相互作用。我们的优势，作为一个跨学科的研究小组，在发展，细胞生理学，神经科学，病毒学和免疫学的基础和应用研究计划，提供了这种仪器的需要的基础。拟议的MPLM系统的添加将为NIH资助的几位关键研究人员提供与之相关的独特能力，我们在轴突运输和神经变性、病毒进入和运输、细胞骨架蛋白重塑、癌症、免疫学方面拥有国家认可的记录。信号转导和分子成像。我们相信，所获得的见解将有可能在艾滋病毒预防、癌症、自身免疫性疾病、糖尿病和神经变性方面开创新的治疗靶点和策略。